A wild-type strain was grown overnight in SD media and diluted to an OD600 of 0.1 in either SD media or SD media containing various concentrations of AZC (1 µg/mL, 2.5 µg/mL, 6 µg/mL, 10 µg/mL). Cells were grown to an OD600 of 1 before being fixed in 3.7% formaldehyde (Sigma). Cells were stained with fluorescein isothiocyanate-ConA, rhodamine-phalloidin, and 4’,6-diamidino-2-phenylindole as described in Ohya et al. (2005) (link) and imaged with 100x magnification on a Zeiss Axio Imager Z1 Fluorescent microscope using ZEN Blue Pro software (Zeiss Inc.). The number of actin patches per cell was measured using CalMorph (v1.2; Ohya et al. 2005 (link)).
Zen blue pro software
Manufactured by Zeiss
ZEN Blue Pro is a comprehensive software suite designed for microscopy image acquisition, processing, and analysis. It provides a user-friendly interface and advanced tools for researchers and scientists working with Zeiss microscopy systems.
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Lab products found in correlation
2 protocols using zen blue pro software
1
Actin Patch Quantification in Yeast Cells
2
Actin Patch Analysis of Yeast Cells
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A wild-type strain was grown overnight in SD media and diluted to an OD600 of 0.1 in either SD media or SD media containing various concentrations of AZC (1 µg/mL, 2.5 µg/mL, 6 µg/mL, 10 µg/mL). Cells were grown to an OD600 of 1 before being fixed in 3.7% formaldehyde (Sigma). Cells were stained with fluorescein isothiocyanate-ConA, rhodamine-phalloidin, and 4',6diamidino-2-phenylindole as described in Ohya et al. (2005) (link) and imaged with 100x magnification on a Zeiss Axio Imager Z1 Fluorescent microscope using ZEN Blue Pro software (Zeiss Inc.). The number of actin patches per cell was measured using CalMorph (v1.2; Ohya et al. 2005) (link).
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