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Galantamine

Manufactured by Yuanye Bio-Technology
Sourced in China

Galantamine is a laboratory equipment product designed for research and analytical purposes. It functions as an acetylcholinesterase inhibitor, which helps to maintain levels of the neurotransmitter acetylcholine in the body. This product is intended for use in various scientific research applications, but its specific intended use should not be extrapolated.

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3 protocols using galantamine

1

Enzyme Assay Protocols for Biochemical Analyses

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Lipase from porcine pancreatic (Type II, EC 3.1.1.3; L3126), α‐amylase from porcine pancreas (Type VI‐B, EC 3.2.1.1, A3176), α‐glucosidase from Saccharomyces cerevisiae (EC 3.2.1.20, G3651), and acetylcholinesterase from Electrophorus electricus (Type VI‐S, EC 3.1.1.7, C3389) were purchased from Sigma‐Aldrich Co., Ltd. Rutin (≥98%), ascorbic acid (≥98%), p‐nitrophenyl palmitate (p‐NPP), 3,5‐dinitrosalicylic acid (DNS), p‐nitrophenyl‐α‐D‐glucopyranoside (α‐p‐NPG), 5,5'‐dithiobis(2‐nitrobenzoic acid) (DTNB), acetylthiocholine iodide, and orlistat were purchased from Hefei Biobomei Biotechnoligy Co., Ltd.. Acarbose was purchased from Nanjing Dulai Biotechnology Co., Ltd.. p‐(Dimethylamino)cinnamaldehyde (p‐DMACA) and galantamine were purchased from Shanghai Yuanye Biotechnology Co., Ltd.. All other chemicals used were of analytical grade and procured from Sinopharm Chemical Reagent Co., Ltd. AB‐8 macroporous resin was purchased from Anhui Sanxing Resin Technology Co., Ltd.
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2

Galantamine and Amyloid-Beta Neuroprotection

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Galantamine (purity > 98%) and peptide amyloid-β (Aβ25–35, purity > 97%) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Acetylcholinesterase (from Electrophorus electricus, 500 units/mg), all-trans astaxanthin standard (purity > 97%), acetylthiocholine iodide (purity > 98%), 5′,5-dithiobis- (2-nitrobenzoic acid) (purity > 98%), analytical grade methanol, ethyl alcohol (purity > 95%), dimethyl sulfoxide, methyl tert-butyl ether, bovine serum albumin, penicillin (100 units/mL), and streptomycin (100 μg/mL) were purchased from Sigma-Aldrich Co (St. Louis, MO, USA). PC12 cell, 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide (MTT), horse serum, and bicinchoninic acid protein assay kit were purchased from Solarbio Science &Technology Co., Ltd. (Beijing, China). RPMI 1640 medium was purchased from Fisher Scientific (Waltham, MA, USA). Fetal bovine serum was purchased from Gemini Bio-Products (West Sacramento, CA, USA). Superoxide dismutase, malondialdehyde, catalase, and acetylcholinesterase (Acetylcholinesterase) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
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3

Fluorometric Assay for Sialidase Activity

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NA from C. perfringens (Type V, 6 UN, lyophilized powder) and 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid sodium salt hydrate (MUNANA) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The BeaverBeads™ Mag NH2 amine-terminated magnetic beads (2 μm, 10 mg/mL) were the products of Beaverbio Co., Ltd. (Suzhou, China). Pyridine, Tris(hydroxymethyl) methyl aminomethane (Tris), 4-morpholineethanesulfonic acid (MES), 50% glutaraldehyde, ammonium acetate, and dimethylsulfoxide (DMSO) were all obtained from Aladdin Chemistry (Shanghai, China). Standard compounds, including galantamine, jatrorrhizine, galuteolin, quercetin, luteolin, and isorhamnetin were obtained from Shanghai Yuanye biotechnology Co., Ltd. (Shanghai, China). Acetonitrile and methanol (HPLC grade) were purchased from Merck (Germany). Deionized water purified by a Milli-Q water-purification system from Millipore (Bedford, MA, USA) was used for reagent and sample preparation and dilution. All buffer mentioned in this article were freshly prepared before experiments.
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