Corticosterone elisa kit

Chicken CRH ELISA kit (Wuhan Colorful Gene Biotechnology Co., Ltd., JYM0155Ch), Corticosterone ELISA kit (Arbor Assays Co., Ltd., K014-H1), Chicken Tumor Necrosis Factor α (TNF-α) ELISA kit (Wuhan Colorful Gene Biotechnology Co., Ltd., JYM0033Ch), and Chicken Factor Related Apoptosis Ligand (FASL) ELISA kit (Shanghai Jianglai Biotechnology Co., Ltd., JL26226) were used to measure CRH, corticosterone, TNF-α and FasL, respectively, in serum and/or follicular fluid. The CRH detection range was 2 to 180 pg/mL (R > 0.92); the minimum level of corticosterone detection was 16.9 pg/mL; TNF-α detection range was 1.2 to 100 pg/mL (R > 0.92); and FasL detection range was 6.25 to 200 pg/mL. To start the measurement, 50 μL of standards or samples were added to wells of a microtiter plate. Then, after experimental procedures were performed in accordance with instructions of the respective kits, the plates were incubated at 37°C for 10 min (corticosterone was incubated at room temperature for 30 min). Finally, the optical density were read at 450 nm wavelength using a plate reader within 15 min after the reaction was terminated by adding 50 μL of the stop solution.