Log In Sign up
The largest database of trusted experimental protocols

Mouse anti gapdh

Manufactured by US Biological
Visit Supplier
Sourced in United States

The Mouse anti-GAPDH is a primary antibody that recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, which is a widely expressed housekeeping gene. This antibody can be used in various immunodetection techniques to identify and quantify GAPDH levels in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti flag m2, by Merck Group (3 mentions) Rabbit anti ha, by Merck Group (2 mentions) Mouse anti control igg, by Santa Cruz Biotechnology (2 mentions) Mouse anti myc 9e10, by Merck Group (2 mentions) Mouse anti gfp, by Merck Group (1 mentions) Mouse anti ha 12ca5, by Santa Cruz Biotechnology (1 mentions) Rabbit anti utx, by Cell Signaling Technology (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Rabbit anti myc, by Merck Group (1 mentions) Rabbit anti flag f7425, by Merck Group (1 mentions) Rabbit anti histone h4, by Active Motif (1 mentions) Anti nap1l1, by Santa Cruz Biotechnology (1 mentions) Rabbit anti irf3, by Cell Signaling Technology (1 mentions) Rabbit anti ets1, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

GAPDH (13 citations) Anti-GAPDH (7 citations)

5 protocols using mouse anti gapdh

1

Plasmid and Antibody Protocol for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following plasmids, antibodies and oligos were used: rabbit anti-HA (6908, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-HA(12CA5) (sc-57592, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-GFP (G1546, Sigma-Aldrich, St. Louis), mouse anti-GAPDH (G8140, US Biological, Salem, MA, USA), mouse anti-ORF59 (gift from Dr. Bala Chandran), rabbit anti-control IgG (sc-2027, Santa Cruz Biotechnology), mouse anti-control IgG (sc-2025, Santa Cruz Biotechnology), rabbit anti-JMJD3 (3457S, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-UTX (33510S, Cell Signaling Technology), control LacZ oligo (Protein and Nucleic Acid Facility, Stanford University), and PAN oligos (Protein and Nucleic Acid Facility, Stanford University). Plasmids pCS2-UTX-Flag and pCS2-JMJD3-Flag were purchased from Addgene.
Hiura K., Strahan R., Uppal T., Prince B., Rossetto C.C, & Verma S.C. (2020). KSHV ORF59 and PAN RNA Recruit Histone Demethylases to the Viral Chromatin during Lytic Reactivation. Viruses, 12(4), 420.
+ Open protocol
+ Expand
2

Antibody Characterization for Molecular Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: mouse anti-Flag (M2, Sigma-Aldrich, St. Louis, MO), rabbit anti-Flag (F7425, Sigma-Aldrich, St. Louis), mouse anti-RTA (mouse hybridoma), mouse anti-LANA (mouse hybridoma), mouse anti-Myc (mouse hybridoma), rabbit anti-HA (6908, Sigma-Aldrich, St. Louis, MO), mouse anti-HA12CA5 (sc-57592, Santa Cruz Biotechnology), mouse anti-GFP (G1546, Sigma-Aldrich, St. Louis), mouse anti-GAPDH (G8140, US Biological, Salem MA), and rabbit anti-Myc (SAB4300605, Sigma-Aldrich, St. Louis, MO), goat anti-PRMT5 (C-20, sc-22132, Santa Cruz Biotechnology), mouse anti-ORF59 (generous gift from Dr. Bala Chandran), rabbit anti-Control IgG (sc-2027, Santa Cruz Biotechnology), mouse anti-Control IgG (sc-2025, Santa Cruz Biotechnology), rabbit anti-H4R3me2s (61187, Active Motif, Carlsbad CA), rabbit anti-Histone H4 (#61299 Active Motif), rabbit-anti-PRMT5 antibody (#61001 Active Motif), rabbit anti-control IgG (ChIP grade—Cell Signaling Technology #2729, rabbit anti-COPR5 antibody (Novus Biologicals #NBP2-30884), and additional rabbit-anti ORF59 antibody custom synthesized for our lab by GenScript.
Strahan R.C., McDowell-Sargent M., Uppal T., Purushothaman P, & Verma S.C. (2017). KSHV encoded ORF59 modulates histone arginine methylation of the viral genome to promote viral reactivation. PLoS Pathogens, 13(7), e1006482.
+ Open protocol
+ Expand
3

Antibody Panel for KSHV Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following commercial antibodies were used for this study: rat anti-LANA (Advanced Biotechnologies, Inc.), mouse anti-GAPDH (US Biological), mouse anti-Flag M2 (Sigma-Aldrich, St. Louis, MO, USA), mouse anti-Myc 9E10 (Sigma-Aldrich, St. Louis, MO, USA), rabbit polyclonal anti-NAP1L1 (Santa Cruz Biotechnology), mouse monoclonal anti-NAP1L1 (Santa Cruz Biotechnology). Mouse monoclonal anti-LANA antibody was generated at GenScript. Mouse anti-ORF59 and rabbit anti-RTA antibodies were generously provided by Dr. Bala Chandran, Rosalind Franklin University of Medicine and Science, Chicago and Dr. Yoshihiro Izumiya, UC Davis, respectively. Mouse anti-K8 antibody was obtained from Dr. Rossetto’s laboratory (University of Nevada, Reno, NV).
Gupta N., Thakker S, & Verma S.C. (2016). KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription. Scientific Reports, 6, 32633.
+ Open protocol
+ Expand
4

Immunological Profiling of Autoantibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-nuclear antibodies including anti-dsDNA, -ssDNA, -nucleosomes or -histones were measured as previously described (22 (link)). Total IgM and IgG isotypes and cytokine/chemokine expression was quantified using Luminex-based assays (Affymetrix). Transcription factor analysis was accomplished using Nuclear Extraction and Procarta Transcription Factor Plex Kits according to manufacturer’s instructions (Affymetrix). Immunoblot analysis was performed as previously described (26 ) and the concentration of the primary antibodies were as follows: rabbit anti-IRF3 (1:1000, Cell Signaling), rabbit anti-IRF7 (1 mg/mL, Abcam) and mouse anti-GAPDH (1:500, US Biological) antibodies.
Cuda C.M., Misharin A.V., Gierut A.K., Saber R., Haines GK I.I.I., Hutcheson J., Hedrick S.M., Mohan C., Budinger G.S., Stehlik C, & Perlman H. (2014). Caspase-8 Acts as a Molecular Rheostat to Limit RIPK1- and MyD88-Mediated Dendritic Cell Activation. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5548-5560.
+ Open protocol
+ Expand
5

Antibody Utilization in Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following commercial antibodies were used: Mouse anti-EGFL7 (sc-373898, Santa Cruz, USA), Rabbit anti-EGFL7 antibody (ab115786, Abcam), mouse anti-Daxx (sc-8043, Santa Cruz), rabbit anti-Ets1 (sc-350, Santa Cruz), mouse anti-GAPDH (US Biological, USA), mouse anti-Flag M2 (Sigma Aldrich, USA) and mouse anti-Myc 9E10 (Sigma-Aldrich). Mouse anti-LANA monoclonal antibody was generated at Genscript (USA).
Thakker S., Strahan R.C., Scurry A.N., Uppal T, & Verma S.C. (2017). KSHV LANA upregulates the expression of epidermal growth factor like domain 7 to promote angiogenesis. Oncotarget, 9(1), 1210-1228.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!