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Anti prdx1

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Anti-PRDX1 is a primary antibody that recognizes the Peroxiredoxin 1 (PRDX1) protein. PRDX1 is a member of the peroxiredoxin family of antioxidant enzymes that play a role in redox regulation of the cell. The Anti-PRDX1 antibody can be used to detect and study the PRDX1 protein.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (3 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Anti gpx4, by Santa Cruz Biotechnology (1 mentions) Anti p rip1, by Cell Signaling Technology (1 mentions) Anti slc3a2, by Santa Cruz Biotechnology (1 mentions) Anti hspb1, by Cell Signaling Technology (1 mentions) Anti p mlkl, by Cell Signaling Technology (1 mentions) Anti slc7a11, by Santa Cruz Biotechnology (1 mentions) Anti zo 1, by Santa Cruz Biotechnology (1 mentions) Diamide, by Merck Group (1 mentions) Catalase, by Merck Group (1 mentions) Crystal violet, by Merck Group (1 mentions) Anti gfp, by Thermo Fisher Scientific (1 mentions) 5 bromo 2 deoxyuridine brdu, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Thermo Fisher Scientific (1 mentions) Anti prdx3, by Abcam (1 mentions) L dehydroascorbic acid, by Merck Group (1 mentions) N ethylmaleimide, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

2 protocols using anti prdx1

1

Western Blot Analysis of Protein Markers

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Samples from tissues or cells were lysed in RIPA buffer supplemented with 1x protease and phosphatase inhibitor cocktail (Thermo Fisher, America) and incubated on ice for 30 min. After centrifugation at 14000 × g for 10 min at 4 °C, the supernatant was collected to determine the protein concentration by BCA assay. The samples were prepared for electrophoresis, followed by transfer onto PVDF membranes. The primary antibodies used in this work were as follows: anti-ZO-1 (Santa Cruz Biotechnology), anti-p-MLKL (Cell Signaling Technology), anti-p-RIP1 (Cell Signaling Technology), anti-cleaved/full-length caspase 9 (Cell Signaling Technology), anti-cleaved/full-length caspase 3 (Cell Signaling Technology), anti-GPX4 (Santa Cruz Biotechnology), anti-SLC3A2 (Santa Cruz Biotechnology), anti-SLC7A11 (Santa Cruz Biotechnology), anti-ANXA2 (ABclonal), anti-His-tag (ABclonal), anti-HA-tag (ABclonal), anti-HSPB1 (Cell Signaling Technology), and anti-PRDX1 (Cell Signaling Technology). Secondary antibodies were purchased from ABclonal. The membranes were visualized via the chemiluminescence method.
He J., Hou X., Wu J., Wang K., Qi X., Wei Z., Sun Y., Wang C., Yao H, & Liu K. (2024). Hspb1 protects against severe acute pancreatitis by attenuating apoptosis and ferroptosis via interacting with Anxa2 to restore the antioxidative activity of Prdx1. International Journal of Biological Sciences, 20(5), 1707-1728.
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2

Oxidative Stress Assay Reagents

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l-ascorbic acid (AA), l-dehydroascorbic acid (DHA), N-ethylmaleimide (NEM), crystal violet, N-acetyl-l-cysteine (NAC), glutathione, DMSO, dithiothreitol (DTT), hydrogen peroxide (H2O2), catalase, diamide, trichloroacetic acid (TCA) and cycloheximide were purchased from Sigma-Aldrich. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA), 5-bromo-2′-deoxy-uridine (BrdU) and propidium iodide (PI) were from Thermo Fisher Scientific. The following antibodies were used: anti-PRDX3 (Abcam), anti-GFP (Life Technologies), anti-β-actin (Sigma-Aldrich), anti-PRDX1 (Cell Signaling Technology), FITC Mouse anti-BrdU (BD Biosciences).
El Banna N., Hatem E., Heneman-Masurel A., Léger T., Baïlle D., Vernis L., Garcia C., Martineau S., Dupuy C., Vagner S., Camadro J.M, & Huang M.E. (2019). Redox modifications of cysteine-containing proteins, cell cycle arrest and translation inhibition: Involvement in vitamin C-induced breast cancer cell death. Redox Biology, 26, 101290.
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