Isopropyl β thiogalactopyranoside iptg

Heterobifunctional polyethylene glycol (NHS-PEG-PDP; 2 kDa) was purchased from JenKem Technology USA Inc. (Plano, TX). E. coli strains (TOP10 and BL21star (DE3)), pEXP-5-NT/TOPO^® TA expression kit, fetal bovine serum (FBS), PBS, Dulbecco's Modified Eagle Medium (DMEM) and Hoechst 33342 were purchased from Invitrogen (Carlsbad, CA). Traut's reagent (2-iminothiolane), Isopropyl-β-thiogalactopyranoside (IPTG) and kanamycin were from Fisher Scientific (Pittsburg, PA). T4 DNA ligase and DNA restriction enzymes (NheI and EcoRI) were purchased from New England Biolabs (Ipswich, MA), and PCR primers were from Integrated DNA Technologies Inc. (Coralville, IA). Rabbit reticulocyte lysate assay kit and luciferase assay kit were purchased from Promega Corporation (Madison, WI), and BCA assay kit was from Bio-Rad Laboratories (Hercules, CA). Melittin (from honey bee venom) and Rhodamine B isothiocyanate (TRITC) were obtained from Sigma-Aldrich (St. Louis, MO). Cell proliferation kit II (XTT) was purchased from Roche Applied Science (Indianapolis, IN).

Isopropyl-β-thiogalactopyranoside (IPTG) and carbenicillin were purchased from Fisher Scientific (Pittsburg, PA). Traut's reagent (2-iminothiolane), heparin sulfate, MES (2-(N-morpholino) ethanesulfonic acid), EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide Hydrochloride) and TRITC (rhodamine B isothiocyanate) were purchased from Sigma-Aldrich (St. Louis, MO). BL21star (DE3) E. coli strain, AcTEV™ protease, Hybridoma serum free medium (SFM), Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum albumin (FBS) and PBS (pH 7.4) were purchased from Invitrogen (Carlsbad, CA). Dylight 488 and Dylight 775-B4 were purchased from Thermo Scientific (Rockford, IL). PEG (NH₂-PEG-MAL; 3.5 kDa) was purchased from JenKem Technology USA Inc. (Allen, TX).