All compounds except for ciclesonide and EIDD-1931 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). ciclesonide and EIDD-1931 were purchased from Cayman Chemical (Ann Arbor, MI, USA). The following are the lot numbers and purities of each compound: aprotinin (lot, 62009; purity of ≥98.0%), bromhexine hydrochloride (lot, 15159; purity of 99.39%), niclosamide (lot, 15718; purity of 98.68%), ciclesonide (lot, 0472344-2; purity of ≥98.0%), remdesivir (lot, 46182; purity of 99.78%), EIDD-2801 (lot, 67548; purity of 99.94%), and EIDD-1931 (lot, 0590872-1; purity of ≥95.0%). Stock solution was dissolved in dimethyl sulfoxide (DMSO) at a 10 mM concentration. Anti‐SARS‐CoV‐2 N protein antibody was purchased from Sino Biological Inc. (Beijing, China). Alexa Fluor 488 goat anti‐rabbit IgG (H + L) secondary antibody and Hoechst 33342 were purchased from Molecular Probes. Paraformaldehyde (PFA) (32% aqueous solution) and normal goat serum were purchased from Electron Microscopy Sciences (Hatfield, PA, USA) and Vector Laboratories, Inc. (Burlingame, CA, USA), respectively.
Eidd 1931
Manufactured by Cayman Chemical
Sourced in United States
EIDD-1931 is a chemical compound used in research and laboratory settings. It serves as a tool for scientific investigation, with its core function being to facilitate chemical and biological experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ciclesonide, by MedChemExpress (2 mentions)
Eidd 1931, by MedChemExpress (2 mentions)
Ciclesonide, by Cayman Chemical (2 mentions)
Anti sars cov 2 n protein antibody, by Sino Biological (2 mentions)
Alexa fluor 488 goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (2 mentions)
Paraformaldehyde pfa 32 aqueous solution, by Electron Microscopy Sciences (2 mentions)
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Normal goat serum (ngs), by Electron Microscopy Sciences (2 mentions)
Celltiter glo viability assay, by Promega (1 mentions)
5 fluorouracil, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
3 protocols using eidd 1931
1
SARS-CoV-2 Anti-Viral Compound Screening
2
SARS-CoV-2 Anti-Viral Compound Screening
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All compounds except for ciclesonide and EIDD-1931 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). ciclesonide and EIDD-1931 were purchased from Cayman Chemical (Ann Arbor, MI, USA). The following are the lot numbers and purities of each compound: aprotinin (lot, 62009; purity of ≥98.0%), bromhexine hydrochloride (lot, 15159; purity of 99.39%), niclosamide (lot, 15718; purity of 98.68%), ciclesonide (lot, 0472344-2; purity of ≥98.0%), remdesivir (lot, 46182; purity of 99.78%), EIDD-2801 (lot, 67548; purity of 99.94%), and EIDD-1931 (lot, 0590872-1; purity of ≥95.0%). Stock solution was dissolved in dimethyl sulfoxide (DMSO) at a 10 mM concentration. Anti‐SARS‐CoV‐2 N protein antibody was purchased from Sino Biological Inc. (Beijing, China). Alexa Fluor 488 goat anti‐rabbit IgG (H + L) secondary antibody and Hoechst 33342 were purchased from Molecular Probes. Paraformaldehyde (PFA) (32% aqueous solution) and normal goat serum were purchased from Electron Microscopy Sciences (Hatfield, PA, USA) and Vector Laboratories, Inc. (Burlingame, CA, USA), respectively.
3
Antiviral Efficacy of 5-FU and EIDD-1931 against LASV
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5’-fluorouracil (Cat# 03738, Sigma) and N4-hydroxycytidine (EIDD-1931) (Cat#9002958, Cayman) were dissolved in DMSO (cell culture grade, Sigma) and further diluted in DMEM media contain 2% FBS and 0.1% DMSO. Vero cells were mock-treated or treated with 5-FU or EIDD-1931 at different concentrations as indicated in each experiment for 4 hrs and then infected with wt rLASV and ExoN- rLASV (MOI 0.1) for 1 hr. Virus inoculums were removed and replaced with fresh media (DMEM + 2% FBS and 0.1% DMSO) containing 5-FU or EIDD-1931 of different concentrations as indicated. At 48 hpi, the supernatants of infected cells were harvested and subjected to plaque assay to determine virus titers. The viability of Vero cells treated with 5-FU and EIDD-1931 alone were assessed using CellTiter-Glo Viability Assay (Promega).
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