Bs1482m

The following primary antibodies were used for immunoblotting analysis: anti-KAT7 (ab190908, Abcam), anti-PKD1 (90039S, Cell Signaling Technology-CST), anti-HA (CST, 3724S), anti-Flag (Sigma, F7425), anti-pMOTIF PKD1 (4381, CST), anti-phosphoserine (ab9332, Abcam), anti-phosphothreonine (CST, 9381S), anti-Tubulin (BS1482M, BioWorld), anti-phospho-PKD1(ser744/748) (CST, 2054), anti-GAPDH (Bioworld, AP0063), anti-Myc, anti-GST(Santa Cruz, sc-965), anti-mcm2 (ab133325, Abcam), anti-mcm6 (ab201683, Abcam), anti-H4 (13919S, CST), anti-H4acK5, K8, K12, K16 (ab177790, Abcam), anti-H3.

Proteins were extracted from HEK 293 T cells or human prefrontal cortex brain tissues using tissue total protein lysis buffer (Beyotime, Shanghai, China) for each assay. Proteins were electrophoretically separated on SDS-PAGE gels and subsequently immunoblotted onto nitrocellulose membranes that were blocked in skim milk and incubated at 4 °C for 16 h using antibodies against GFP (1:5000, ab290, Abcam), β-tubulin (1:10,000, BS1482M, Bioworld), TARP γ-8 (1:500, abs132666a, Absin) and GAPDH (1:10,000, 60,004–1-Ig, Proteintech). Detection was carried out using the horseradish peroxidase-conjugated secondary antibodies goat anti-mouse IgG (1:10,000, BS12478, Bioworld) and goat anti-rabbit IgG (1:15,000, BS13278, Bioworld) along with a High-sig ECL kit (Tanon, Shanghai, China), and signals were recorded using a Tanon 5200 multi automatic chemiluminescence image analysis system (Tanon, Shanghai, China). For western blot quantitation, images were analyzed using ImageJ and expressed as arbitrary units (AU). GFP protein expression was normalized to β-tubulin protein expression, and TARP γ-8 expression was normalized to GAPDH expression.