1H NMR and 13C NMR spectra were recorded in CDCl3 by using a Bruker DRX Avance (300 and 75 MHz) spectrometer equipped with a nonreverse probe. Chemical shifts [ppm] are referenced to the residual solvent proton/carbon signal. Polarimetry analyses were performed with a PerkinElmer 343 Plus equipped with Na/Hal lamp. MS analyses were performed by using a Thermo Finnigan (MA, USA) LCQ Advantage system MS spectrometer with an electrospray ionization source and an ion‐trap mass analyzer. Mass spectra were obtained by direct infusion of a sample solution in MeOH under ionization (ESI+). Catalytic reactions were monitored by HPLC analysis with a Merck‐Hitachi L‐7100 equipped with Detector UV6000LP and a chiral column (AD, OJ‐H Chiralcel, Lux Cellulose‐4, Lux Cellulose‐2 or Lux Amylose‐2).
343 plus
Manufactured by PerkinElmer
The 343 Plus is a high-performance liquid chromatography (HPLC) detector developed by PerkinElmer. It is designed to provide accurate and reliable detection of a wide range of analytes in various applications. The 343 Plus utilizes advanced technology to deliver consistent results, ensuring the quality and integrity of analytical data.
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2 protocols using 343 plus
1
Spectroscopic and Chromatographic Analysis
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1H NMR and 13C NMR spectra were recorded in CDCl3 by using a Bruker DRX Avance (300 and 75 MHz) spectrometer equipped with a nonreverse probe. Chemical shifts [ppm] are referenced to the residual solvent proton/carbon signal. Polarimetry analyses were performed with a PerkinElmer 343 Plus equipped with Na/Hal lamp. MS analyses were performed by using a Thermo Finnigan (MA, USA) LCQ Advantage system MS spectrometer with an electrospray ionization source and an ion‐trap mass analyzer. Mass spectra were obtained by direct infusion of a sample solution in MeOH under ionization (ESI+). Catalytic reactions were monitored by HPLC analysis with a Merck‐Hitachi L‐7100 equipped with Detector UV6000LP and a chiral column (AD, OJ‐H Chiralcel, Lux Cellulose‐4, Lux Cellulose‐2 or Lux Amylose‐2).
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1H NMR and 13C NMR spectra were recorded in CDCl3 by using a Bruker DRX Avance (300 and 75 MHz) spectrometer equipped with a nonreverse probe. Chemical shifts [ppm] are referenced to the residual solvent proton/carbon signal. Polarimetry analyses were performed with a PerkinElmer 343 Plus equipped with Na/Hal lamp. MS analyses were performed by using a Thermo Finnigan (MA, USA) LCQ Advantage system MS spectrometer with an electrospray ionization source and an ion‐trap mass analyzer. Mass spectra were obtained by direct infusion of a sample solution in MeOH under ionization (ESI+). Catalytic reactions were monitored by HPLC analysis with a Merck‐Hitachi L‐7100 equipped with Detector UV6000LP and a chiral column (AD, OJ‐H Chiralcel, Lux Cellulose‐4, Lux Cellulose‐2 or Lux Amylose‐2).
2
Elucidation of Sesquiterpenes and Alkaloids via Chromatography
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For column chromatography (CC), Si-gel (107734, 107741, and 107749, Merck) and Sephadex LH-20 (Sigma–Aldrich) were used. For TLC chromatography, Si-gel (105554 and 105715; Merck) plates were used and visualized with óleum solution (sesquiterpenes) and Dragendorff’s reagent (alkaloids). The prep. HPLC chromatography was carried out on a Beckman 125P system equipped with an Ultrasphere semiprep column (10 × 250 mm) and a UV/visible diode array detector 168. Optical rotations were determined at 20 °C on a Perkin-Elmer 343 Plus polarimeter. IR Spectra were recorded in CHCl3 on a Perkin Elmer 1600 spectrophotometer. NMR spectra were recorded on a pulsed-field gradient Bruker Advance II-500 MHz spectrometer (solvent as internal standard CDCl3, at δH 7.26 and δC 77.0) and the Bruker software was used for DEPT, 1H, 1H-COSY (Homonuclear correlation spectroscopy), NOESY (Nuclear Overhauser Effect Spectroscopy), HSQC (Heteronuclear single quantum coherence spectroscopy) and HMBC (Heteronuclear Multiple Bond Correlation). EI and HR-EI-MS spectra were recorded in m/z on a Micromass Autospec spectrometer.
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