Anti brg1

We used haematoxylin‐eosin (HE) staining and immunohistochemical staining to assess the pathological changes and the expression of Brg1, BrdU and Ki‐67, respectively, in paraffin‐embedded sections of mouse livers. For Brg1, BrdU and Ki‐67 immunohistochemistry staining, the slices were incubated with anti‐Brg1 (1:200; Abcam), anti‐BrdU (1:400; Abcam) and anti‐Ki‐67 (1:500; Abcam) overnight at 4°C, followed by biotinylated secondary antibody at 37°C for 1 hour. Colour development was carried out with DAB (3,3‐diaminobenzidine) and running water for 5 minutes. The slides were counterstained with 1% Mayer's haematoxylin. Brg1, BrdU and Ki‐67 immunostaining were scored and examined by two independent assessors. KO group and WT group were used for immunohistochemical detection (n = 5).

10% trichloroacetic acid was used to prepare WCE by incubation on ice for 30 minutes. The WCEs were re-suspended in 500 μL of SDS loading dye. 10 μL of 1 N NaOH was added to neutralize the final solution, if required. WCEs were subjected to electrophoresis through 10% SDS-PAGE. Proteins transferred to a nitrocellulose membrane were blocked in 5% skim milk and probed with the indicated antibodies. Antibodies and dilutions used were anti-Flag (1:4000; Sigma), anti-Actin (1:10000; Abcam), anti-Brg1 (1:1000, as described^{66 (link)})

The ChIP assays were performed using ChIP kit (Catalog no. 17-371; Millipore). The procedure was according to the kit instruction manual provided by the manufacturer. Briefly, 1 × 10⁷ Myc-CaP cells were fixed by 1% formaldehyde, fragmented by sonication to shear the chromatin to 400–1000 bp. The sheared crosslinked chromatin was incubated with IgG, anti-BRG1 (Abcam, clone: EPNCIR111A, ab110641), anti-BAF155 (Santa Cruz Biotechnology; clone: G-7, sc-365543X), anti-ARID1A (Cell Signaling Technology; clone: D2A8U, 12354 S) and anti-ARID1B (Cell Signaling Technology; clone: E9J4T, 92964 S) antibodies (10 μg antibody for each ChIP reaction) overnight followed by Protein G conjugated agarose beads incubation. The precipitated DNA was amplified by primers and quantified by QuantStudio 7 Flex Real Real-Time PCR System (Applied Biosystems). ChIP primer sequences can be found in the Supplementary Table 3.

The protein expression levels of BRG1, Nu-Nrf2, HO-1, Keap-1 and caspase-3 were measured by Western blotting. Generally, total proteins were extracted from NCM460 cells and mouse IECs using RIPA buffer containing protease inhibitors (Roche, Basel, Switzerland), and nuclear proteins were extracted from cells with a nuclear protein extraction kit (Beyotime, Shanghai, China). Then, the protein concentrations were detected with a BCA protein assay kit (Beyotime, Shanghai, China). 10% SDS-PAGE was used to separate equal amount of proteins (40 μg) and transferred onto a PVDF membrane (Millipore, Boston, MA, USA) by electroblotting. The membrane was then blocked with 5% BSA and incubated with the appropriate primary antibodies, including anti-BRG1 (1:1000, Abcam, Cambridge, U.K), anti-Nrf2 (1:1000, CST, Danvers, MA, USA), anti-HO-1 (1:1000, CST, Danvers, MA, USA), anti-Keap-1 (1:1000, Abcam, Cambridge, U.K.), anti-β-actin (1:1000, Beyotime, Shanghai, China), and anti-Lamin B2 (1:1000, CST, Danvers, MA, USA) at 4°C overnight. Afterward, the membrane was incubated with secondary antibodies (1:5000; Beyotime, Shanghai, China) conjugated with peroxidase for 1 h at room temperature. The proteins were detected by an enhanced chemiluminescence (ECL) kit (Beyotime, Shanghai, China). Protein expression was analyzed using Image J software.

The following antibodies were used in the study: anti-BAF155 (Abcam, Cambridge, UK, ab172638, IP and Western blotting), anti-BAF170 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA sc166537, Western blotting), anti-BRD9 (Active Motif, Carlsbad, CA, USA 61537, IP and Western blotting), anti-BRD9 (Bethyl Laboratories, Inc., Montgomery, TX, USA, A303-781A, immunocytochemistry), anti-BRD9 (ThermoFisher, customized using porcine BRD9 peptide sequence, IP and Western blotting), anti-BRG1 (Abcam, ab110641, IP and Western blotting), anti-GLTSCR1 (Santa Cruz, sc51508, IP, Western blotting, and immunocytochemistry), anti-SNF5 (Abcam, ab88589, immunocytochemistry), mouse IgG (Abcam, ab6708, IP and Western blotting), rabbit IgG (Abcam, ab171870, IP and Western blotting), mouse IgGκ BP-HRP (Santa Cruz, sc516102, Western blotting), goat anti-rabbit IgG (H + L) (ThermoFisher (Invitrogen), A27036, Western blotting), goat anti-rabbit IgG-TRITC (Sigma, T6778, immunocytochemistry), and anti-rabbit IgG (whole molecule)-FITC (Sigma, F0382).