Pvp 40

The plastic films used in this work include polyethylene terephthalate (PET), polyimide (PI), polytetrafluoroethylene (PTFE), polyvinylpyrrolidone (PVP, Sigma-Aldrich/PVP40), polyvinyl butyral (PVB, Sigma/P110010), polyethylene oxide (PEO, Mv = 1,000,000, Sigma-Aldrich), polyvinylidene fluoride (PVDF), poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP), polylactic acid (PLA), polyethersulfone (PES) filter membrane, weighing paper, aluminum (Al) foil and copper (Cu) foil. Among them, PI, PET, PTFE, PES filter membrane, weighing paper, Al foil and Cu foil are commercial films with different thicknesses. The PVP, PVB, PEO, PVDF, and PVDF-HFP films were fabricated from their solution (4 wt%, PVP and PEO in water; PVB in isopropyl alcohol, PVDF and PVDF-HFP in N-Methyl-2-pyrrolidone) through solvent volatilization. The PLA belt was fabricated via hot drawing with PLA at 250 °C as the sample for shape morphing at the microscale.
The stress-strain curves of these films were obtained by a mechanical tester (C42, MTS Systems Corporation) at 30 mm/min (Supplementary Fig. 2). Stress-strain hysteresis loops of plastic films were conducted at different strains (0.001, 0.0025, 0.005, 0.0075, 0.01, 0.015, 0.02, 0.03, 0.04, 0.06, 0.08, and 0.10) to get the plastic strain-strain curves (Supplementary Fig. 3).

HAuCl₄·3H₂O, K₂CO₃, Immunoglobulin (IgG), PVP-40, BSA, NaCl, Tween-20, EDTA, NaN₃, Sucrose, Tris, β-cyclodextrin, and staphylococcal protein A (SPA) were purchased from Sigma (St. Louis, MO, USA). A lipopolysaccharide extraction and purification kit was purchased from Intron Biotechnology Company (Chengnan, Korea). The water used in this study was deionized. The cover, detection box, and nitrocellulose filter membrane (NC) were all purchased from Advanced Microdevices (Haryana, India).

Zona pellucida were removed from embryos with 3-4 min pronase treatment [0.5% w/v proteinase K (Sigma-Aldrich, P8811) in H-KSOM supplemented with 0.5% PVP-40 (Sigma-Aldrich, P0930)] at 37°C. Subsequently, embryos were incubated in serum containing anti-mouse antibody (Cedarlane, CL2301; lot no. 049M4847V) diluted 1:3 with KSOM for 30 min at 37°C. Following three brief washes in H-KSOM, embryos were incubated in guinea pig complement (Sigma-Aldrich, 1639; lot no. SLBX9353) diluted 1:3 with KSOM for 30 min at 37°C. Lysed outer cells were removed by mouth-pipetting with a narrow glass capillary to isolate the inner cells.

The sample pad is a chopped glass fiber pad cut to 14 mm width (Ahlstrom-Munksjö, Helsinki, Finland, product 8964). The pad is blocked in 0.05% one-day aged casein (from 1% stock), 5 mM borate, 0.2% w/v sucrose, 0.05% w/v PVP-40 (Sigma, St Louis, MO, PVP-40) and 1 mg/mL HBR-1 (Scantibodies, Santee, CA). The sample pad was submerged completely in blocking solution with continuous rocking for 15 minutes and then dried at 25°C in a forced-air oven.

CaM, GST-GluN2B-C and CaMKIIα were purified after bacterial or baculovirus/Sf9 cell expression, as described in detail elsewhere [9] (link), [26] (link), [27] . Pipes, Tween-20, CaCl₂, MgCl₂, EGTA, ADP, Ponceau S, dextran-10 and −70, PVP-40, non-specific rabbit IgG (catalog number: I5006, lot SLBD3695V), chicken lysozyme (catalog number: L7651, lot SL07134), and BSA (catalog number: A2153, lot SLBC8307) were obtained from Sigma. NaCl and anti-GST coated microtiter plates were obtained from Thermo-Fisher. Staurosporine was purchased from LC labs. ATP was purchased from Calbiochem.