Huwe1 a300 486a

Manufactured by Fortis Life Sciences

HUWE1 (A300-486A) is a protein-specific antibody used for the detection and analysis of the HUWE1 protein in various samples and applications. The core function of this product is to provide a reliable tool for researchers to study the HUWE1 protein and its role in cellular processes.

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Lab products found in correlation

Ar sc 816, by Santa Cruz Biotechnology (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Ubiquitin sc 9133, by Santa Cruz Biotechnology (2 mentions) Jmjd1a a301 539a or 538b, by Fortis Life Sciences (2 mentions) Ar 06 680 or h3k9me2 07 441, by Merck Group (2 mentions) 7425 and actin a5441, by Merck Group (2 mentions) Odyssey scanner, by LI COR (1 mentions) Hdac2 sc 9959, by Santa Cruz Biotechnology (1 mentions) Immobilon fl membrane, by Merck Group (1 mentions) P53 sc 6243, by Santa Cruz Biotechnology (1 mentions) Brca1 sc 6954, by Santa Cruz Biotechnology (1 mentions) Ki 67 ab8191, by Abcam (1 mentions) Jmjd1a 12835 1 ap, by Proteintech (1 mentions) Actin a5441, by Merck Group (1 mentions) Etoposide, by Merck Group (1 mentions) Acetyl histone h3, by Merck Group (1 mentions) Active caspase 3 9661, by Cell Signaling Technology (1 mentions)

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HUWE1 (5 citations)

4 protocols using huwe1 a300 486a

Antibody Validation for Cell Analysis

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Antibodies to AR (sc-816), ubiquitin (sc-9133), HA (sc-805), GFP (sc-8334 or sc-9996), myc (sc-789), tubulin (sc-8035) (all from Santa Cruz), JMJD1A (A301-539A or 538B) or HUWE1 (A300-486A) (Bethyl Laboratories), AR (06-680) or H3K9me2 (07-441) (EMD Millipore), c-Myc (9402), p53 (9282), PARP (9542), active caspase-3 (9661) (all from Cell Signaling), and Flag (F7425) and actin (A5441) (Sigma) were used according to the manufacturers’ recommendations.

Fan L., Peng G., Sahgal N., Fazli L., Gleave M., Zhang Y., Hussain A, & Qi J. (2015). Regulation of c-Myc expression by the histone demethylase JMJD1A is essential for prostate cancer cell growth and survival. Oncogene, 35(19), 2441-2452.

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Antibody Validation for Cell Analysis

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Western Blot Analysis of Cell Extracts

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Whole cell extracts were either separated on a 7% Tris-Acetate polyacrylamide (PA) gel (HUWE1, BRCA1) or on a 10% Tris-Glycine PA gel and transferred to an Immobilon-FL membrane (Millipore) for subsequent immunoblotting. Primary antibodies were detected using infrared (IR) Dye-conjugated secondary antibodies (Rockland). The signal was visualized using direct IR fluorescence via the Odyssey Scanner, LI-COR Biosciences. Primary antibodies: BRCA1 (sc-6954, Santa Cruz), Cdc6 (ab155759, Abcam), c-Myc (sc-789, Santa Cruz), HDAC2 (sc-9959, Santa Cruz), HUWE1 (A300-486A, Bethyl Laboratories), p53 (sc-6243, Santa Cruz), Polλ (A301-640A-1, Bethyl Laboratories), TopBP1 (NB100-217, Novus Biologicals), Tubulin (T9026, Sigma-Aldrich). All original images of immunoblots are depicted in Supplementary Fig. S1.

Bosshard M., Aprigliano R., Gattiker C., Palibrk V., Markkanen E., Backe P.H., Pellegrino S., Raymond F.L., Froyen G., Altmeyer M., Bjørås M., Dianov G.L, & van Loon B. (2017). Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Scientific Reports, 7, 15050.

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Antibody Sources for DNA Damage Research

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Antibodies were purchased from the following companies: JMJD1A (12835-1-AP) was from Proteintech (Rosemont, IL). HUWE1 (A300-486A) was from Bethyl Laboratories (Montgomery, TX). RNF8 (sc-271462), BRCA1 (sc-6954), p300 (sc-585), ubiquitin (sc-6085), GFP (sc-8334), HA (sc-7392) and c-Myc (sc-789, sc-40) were from Santa Cruz Biotechnology (Dallas, TX). c-Myc (ab56), PRKDC (ab32566) and Ki67 (ab8191) were from Abcam (Cambridge, UK). NBS1 (3002), p-ATM (4526), ATM (2873), p-Chk2 (2197), Chk2 (2662), XRCC6 (4588), and active caspase-3 (9661) were from Cell Signaling (Danvers, MA). γ-H2AX (05-636), ubiquitin (FK2, ST1200), 53BP1 (MAB3802), Rad51 (ABE257), H3K9me2 (07-441) and acetyl-histone H3 (06-599) were from EMD Millipore (Burlington, MA). Flag (F7425, F3165) and actin (A5441) were from Sigma-Aldrich (St. Louis, MO). Etoposide was from Sigma-Aldrich (St. Louis, MO). Olaparib was from MedChemExpresss (Monmouth Junction, NJ).

Fan L., Xu S., Zhang F., Cui X., Fazli L., Gleave M., Clark D.J., Yang A., Hussain A., Rassool F, & Qi J. (2020). Histone demethylase JMJD1A promotes expression of DNA repair factors and radio-resistance of prostate cancer cells. Cell Death & Disease, 11(4), 214.

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