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The CRL-6361 is a laboratory instrument designed for cell culture applications. It functions as an incubator, providing a controlled environment for the growth and maintenance of cells in vitro. The device regulates temperature, humidity, and gas composition (such as CO2 levels) to support optimal cell culture conditions. This equipment is intended for use in research and diagnostic settings where the cultivation of cells is required.

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Lab products found in correlation

Nih3t3, by American Type Culture Collection (1 mentions) Crl 2638, by American Type Culture Collection (1 mentions) Crl 1658, by American Type Culture Collection (1 mentions)

2 protocols using crl 6361

1

Culturing Murine and Human Colorectal Cancer Cell Lines

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A murine CRC cell line, colon 26 (CRL-2638; American Type Culture Collection (ATCC), Manassas, VA, USA) and a human CRC cell line, KM12C (a gift from Dr. I. J. Fidler, M.D. Anderson Cancer Center) were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS). A mouse fibroblast cell line, NIH3T3 (CRL-6361; ATCC) was maintained in Dulbecco's modified Eagle's medium supplemented with 10% FBS. These cells were cultured at 37°C under 5% CO2.
Tanabe Y., Sasaki S., Mukaida N, & Baba T. (2016). Blockade of the chemokine receptor, CCR5, reduces the growth of orthotopically injected colon cancer cells via limiting cancerassociated fibroblast accumulation. Oncotarget, 7(30), 48335-48345.
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2

Cell Culture Conditions Optimization

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HBEC3 cells and stable cell lines were grown in KSFM media supplemented with bovine pituitary extract and recombinant human EGF unless specific indicated. NIH3T3 cells (CRL-1658, ATCC), Kras-transformed NIH3T3 cells (CRL-6361, ATCC) and HepG2 cells were grown in DMEM media with 10% FBS. H1299 cells were grown in RPMI-1640 media with 10% FBS.
Wu B.K, & Brenner C. (2014). Suppression of TET1-Dependent DNA Demethylation is Essential for KRAS-Mediated Transformation. Cell reports, 9(5), 1827-1840.
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