The detailed procedures were described previously.31 Rabbit monoclonal anti‐YAP/TAZ (D24/E4), rabbit polyclonal anti‐phospho‐YAP (Ser127), and rabbit polyclonal anti‐AJUBA antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse mAb for α‐tubulin was from Sigma‐Aldrich (St. Louis, MO, USA). The corresponding HRP‐conjugated secondary antibodies were used.
Rabbit polyclonal anti phospho yap ser127
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit polyclonal anti‐phospho‐YAP (Ser127) is a laboratory reagent used to detect the phosphorylation of the YAP protein at serine 127 residue. It is a primary antibody produced in rabbits and can be used in various immunoassay techniques, such as Western blotting, to study the activation and regulation of the Hippo signaling pathway.
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2 protocols using rabbit polyclonal anti phospho yap ser127
1
Immunoblotting of YAP/TAZ and AJUBA
2
Quantifying YAP/Lamin A/C Phosphorylation
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Protein lysates from control/DMSO and VP (YAP inhibitor) were separated by electrophoresis on 4–12% bis-Tris SDS PAGE gels, with MOPS buffer and transferred to a nitrocellulose membrane. After blocking, the membranes were incubated overnight for 10 hours at 4°C with the primary antibodies: Rabbit Polyclonal anti-phospho YAP (ser127) (Cell Signaling, #4911S) at 1:1000 dilution or Rabbit Monoclonal anti-phospho Lamin A/C (Ser22) (D2B2E) (Cell Signaling, #13448S) at 1:1000, Rabbit Monoclonal anti-YAP (D8H1X) XP® (Cell Signaling, #14074S at 1:1000, Mouse Monoclonal anti-Lamin A/C (4C11) (Cell Signaling, #4777S) at 1:1000, and Rabbit Monoclonal anti-GAPDH (Cell Signaling, #2118S) at 1:1000. These primary antibodies were detected using Polyclonal Goat anti-Rabbit coupled to IRDye 680RD (LI-COR Bioscences, #925–68071) at 1:10000 or Polyclonal Goat anti-Mouse coupled to IRDye 680RD (LI-COR, #925–68070) at 1:10000.
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