Conditional E2A-PBX1 transgenic mice were reported previously (5 (link)). Transgenic CD19.Cre (Jackson laboratory, (6 (link))), Mb1.Cre (provided by Dr. David Allman, University of Pennsylvania, Philadelphia, PA, USA; and by Dr. Michael Reth, University of Freiburg, Germany (7 (link))) and Mx1.Cre (The Jackson Laboratory (8 (link))) mice were intercrossed to generate E2A-PBX1/CD19.Cre, E2A-PBX1/Mb1.Cre and E2A-PBX1/Mx1.Cre mice respectively, on a C57BL/6 background. Leukemia cells derived from E2A-PBX1/CD19.Cre and E2A-PBX1/Mx1.Cre mice, which were preBCR+ as seen by cytoplasmic μ chain, were used for in vitro and in vivo experiments. Leukemia cells derived from E2A-PBX1/Mb1.Cre mice, which were preBCR-, were used for in vitro experiments. Disease-free survival was defined when showing signs of illness including general lymphadenopathy, lethargy, weight loss and shivering. Moribund mice were euthanized.
Mb1 cre
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
Mb1-Cre is a Cre recombinase expressed under the control of the Mb-1 gene promoter. The Mb-1 gene encodes the B-cell-specific CD79a protein, which is essential for B-cell development and function. Mb1-Cre allows for Cre-mediated recombination specifically in B cells.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6, by Jackson ImmunoResearch (3 mentions)
Nod cg prkdcscid il2rgtm1wjl szj, by Jackson ImmunoResearch (3 mentions)
Nr4a1 mice, by Jackson ImmunoResearch (2 mentions)
Cd8 cre, by Jackson ImmunoResearch (2 mentions)
Cd45.1 boyj mice, by Charles River Laboratories (2 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (2 mentions)
Stat5fl fl, by Jackson ImmunoResearch (2 mentions)
Ptpn6fl fl, by Jackson ImmunoResearch (2 mentions)
Cd19 cre, by Jackson ImmunoResearch (1 mentions)
Mx1 cre, by Jackson ImmunoResearch (1 mentions)
Cd4 cre b6 cg tg cd4 cre 1cwi bfluj, by Jackson ImmunoResearch (1 mentions)
Mrl lpr, by Jackson ImmunoResearch (1 mentions)
Granzyme b cre, by Jackson ImmunoResearch (1 mentions)
Cd4 cre, by Jackson ImmunoResearch (1 mentions)
Cd21 cre, by Jackson ImmunoResearch (1 mentions)
9 protocols using mb1 cre
1
Transgenic Mouse Models of E2A-PBX1 Leukemia
2
Genetically Engineered Mouse Models for Immunological Research
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C57BL/6J, CD4cre (B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ), Mb1cre (B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ), MRL/lpr (MRL/MpJ-Faslpr/J), SLE123 (B6;NZM-Sle1NZM2410/Aeg Sle2NZM2410/Aeg Sle3NZM2410/Aeg/LmoJ), and Rosa26eYFP (B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J) mice were obtained from The Jackson Laboratory (Bar Harbor, ME). T-bet-creERT2 mice were generated by Dr. Lin Gan at the University of Rochester, Rochester, NY. Adora2aflox (B6;129-Adora2atm1Dyj/J) mice were provided by Dr. Joel Linden, La Jolla Institute for Immunology, La Jolla, CA. All mice, except for the MRL/MpJ-Faslpr/J, were on the C57BL/6J background. All mice were housed and bred in the SUNY Upstate Medical University Animal Care Facility (Syracuse, NY), in accordance with institutional guidelines for animal welfare. All mice used for experiments were at least 6 weeks old, and both male and female mice were used unless otherwise stated. All studies involving animals were approved by the SUNY Upstate Medical University Institutional Animal Care and Use Committee NYSDOH Unit A073 IACUC number 311.
3
Conditional Knockout Mouse Models for IL-27 Signaling
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C57BL/6 WT, conditional KO, and germline KO mice were used for this study. IL-27p28-eGFP mice were generated by Ross Kedl, University of Colorado Anschutz, Aurora, CO, and generously gifted to us by Ross Kedl and Zhenming Xu, University of Texas at San Antonio, San Antonio, TX (39 (link)). IL-27p28flox/flox were kindly provided by Li-Fan Lu, University of California San Diego, La Jolla, CA (40 (link)). The generation and characterization of IL-27raflox/flox was described previously (41 (link)). CD45.1+ GP66–77 T cell receptor tg (SMARTA), Ebi3−/−, WSX-1−/−, and Cre-expressing lines were purchased from The Jackson Laboratory. To obtain Il27ra−/− SMARTA CD45.1+ mice, WSX-1−/− mice were crossed to CD45.1+ SMARTA mice. To generate conditional deletion of IL-27p28flox/flox and IL-27raflox/flox, mice were crossed to Cre-expressing lines obtained from The Jackson Laboratory: Mb1-Cre to target B cells, granzyme B-Cre to target activated T cells, and CD4-Cre to target T cells. All mice were bred and maintained under specific pathogen-free conditions.
4
Genetic Mouse Models for Nr4a1 and Nr4a3
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Nr4a1–/–, Nr4a1fl/fl, and Nr4a3–/– mice were previously described (6 (link), 20 (link), 23 (link)). Nr4a1fl/fl were previously obtained from Catherine Hedrick (La Jolla Institute for Immunology, La Jolla, California, USA) with permission from Pierre Chambon (University of Strasbourg, Strasbourg, France; ref. 6 (link)). Nr4a1–/– mice were obtained from The Jackson Laboratory, and this line is used throughout the manuscript exclusively as single germline knockout comparator (23 (link)). Nr4a3–/– mice were generated in our laboratory as previously described (20 (link)). CD8-cre and mb1-cre were obtained from The Jackson Laboratory (38 (link), 58 (link)). C57BL/6 mice were from The Jackson Laboratory, and CD45.1+ BoyJ mice were from Charles River Laboratories. To generate gDKO Nr4a1–/– Nr4a3–/– mice, we bred Nr4a3–/– and Nr4a1fl/fl mice with germline recombination of the loxp-flanked locus and confirmed loss of exon 2 by genomic DNA PCR and transcript quantitative PCR. All strains were fully backcrossed to C57BL/6 genetic background for at least 6 generations. Mice of both sexes were used for experiments between the ages of 3 and 10 weeks except for BM chimeras as described below.
5
Generation and Use of Transgenic Mouse Models
6
Genetically Engineered Mouse Models
7
Generating IRF4-Deficient Mice
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IRF4fl/fl (14 (link)) and CD21-cre (20 (link)) mice were obtained from Jackson Labs (catalog numbers 009380, and 006368, respectively) and crossed to each other to generate mice with IRF4 deleted in mature B cells. We also attempted to generate mice lacking IRF4 in all B cells using mb1-cre (Jackson Labs catalog number 020505). However, these studies were unsuccessful because, the mb1-cre caused frequent deletion of IRF4 in the germline as has been described for some loci (21 (link)). These germline deleted mice were bred to establish the IRF4-deficient mice. Mice are on the C57BL/6 background. Mice were sex and age matched and littermate controls were used whenever possible. All experiments were approved by the University of Texas Southwestern Medical Center Institutional Animal Care and Use Committee.
8
Generation and Use of Transgenic Mouse Models
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C57BL/6, NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ, Mb1-Cre, Stat5fl/fl, and Ptpn6fl/fl mice were purchased from The Jackson Laboratory. Mapk1fl/fl and Blnk−/− mice were obtained from Dr. Martin McMahon and Dr. Hassan Jumaa, respectively. MyceGFP/+ mice (Huang, C.Y. et al. 2008) were crossed with Bcl6mCherry/+ mice (Geng, H. et al. 2015) to generate MyceGFP/+; Bcl6mCherry/+ reporter mice. For animals bred in house, littermates of the same sex were randomized to experimental groups. All mouse breeding and experiments were subject to institutional approval by the Beckman Research Institute Animal Care and Use Committee. We have complied with all relevant ethical regulations. All mouse models used in the study are listed in Supplementary Table S7 .
9
Generating Nr4a1 and Nr4a3 Knockout Mice
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Mice. Nr4a1 -/-, Nr4a1 fl/fl , and Nr4a3 -/-mice were previously described (14, 27, 30) .
Nr4a1 fl/fl were previously obtained from Catherine Hedrick (La Jolla Institute for Immunology) with permission from Pierre Chambon (University of Strasbourg) (14) .
Nr4a1 -/-mice were obtained from The Jackson Laboratory and this line is used throughout the manuscript exclusively as single germline knockout comparator (30), Nr4a3 -/-mice were generated in our laboratory as previously described (27) . CD8-cre and mb1-cre were obtained from The Jackson Laboratory (46, 77) . C57BL/6 mice were from The Jackson Laboratory and CD45.1 + BoyJ mice were from Charles River Laboratories. To generate germline DKO Nr4a1 -/-Nr4a3 -/-mice, we bred Nr4a3 -/-and Nr4a1 fl/fl mice with germline recombination of the loxp-flanked locus, and confirmed loss of exon 2 both by genomic DNA PCR and transcript qPCR. All strains were fully backcrossed to C57BL/6 genetic background for at least 6 generations. Mice of both sexes were used for experiments between the ages of 3 and 10 weeks except for BM chimeras as described below. All mice were housed in a specific pathogen-free facility at UCSF according to the University and National Institutes of Health guidelines.
Nr4a1 fl/fl were previously obtained from Catherine Hedrick (La Jolla Institute for Immunology) with permission from Pierre Chambon (University of Strasbourg) (14) .
Nr4a1 -/-mice were obtained from The Jackson Laboratory and this line is used throughout the manuscript exclusively as single germline knockout comparator (30), Nr4a3 -/-mice were generated in our laboratory as previously described (27) . CD8-cre and mb1-cre were obtained from The Jackson Laboratory (46, 77) . C57BL/6 mice were from The Jackson Laboratory and CD45.1 + BoyJ mice were from Charles River Laboratories. To generate germline DKO Nr4a1 -/-Nr4a3 -/-mice, we bred Nr4a3 -/-and Nr4a1 fl/fl mice with germline recombination of the loxp-flanked locus, and confirmed loss of exon 2 both by genomic DNA PCR and transcript qPCR. All strains were fully backcrossed to C57BL/6 genetic background for at least 6 generations. Mice of both sexes were used for experiments between the ages of 3 and 10 weeks except for BM chimeras as described below. All mice were housed in a specific pathogen-free facility at UCSF according to the University and National Institutes of Health guidelines.
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