Antibodies were obtained from the following sources: anti-raptor, -rictor, and -mSIN1 antibodies were from Bethyl Laboratories (Montgomery, TX, USA); MF20 anti-sarcomeric MHC and F5D anti-myogenin antibodies were from the Developmental Studies Hybridoma Bank (developed under the auspices of the National Institute of Child Health and Human Development, National Institutes of Health, and maintained by the Department of Biological Sciences, University of Iowa, Iowa City, IA, USA); anti-DEPTOR were from Novus (Littleton, Colorado, USA). All other primary antibodies were from Cell Signaling Technology (Danvers, MA, USA). All secondary antibodies were from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA). 1,2-dioctanoyl-sn-glycero-3–PA was obtained from Avanti Polar Lipids (Alabaster, AL, USA). Akti1/2 were from Calbiochem-Merck (Darmstadt, Germany). Mouse PLD2 shRNA were previously described16 (link). All other reagents were from Sigma-Aldrich (St. Louis, MO, USA).
Anti raptor
Manufactured by Fortis Life Sciences
Sourced in United States
The Anti-raptor is a laboratory equipment designed to provide a controlled environment for cell culture applications. Its core function is to maintain consistent temperature, humidity, and atmospheric conditions to support the growth and proliferation of cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Akti 1 2, by Merck Group (1 mentions)
Rictor, by Fortis Life Sciences (1 mentions)
1 2 dioctanoyl sn glycero 3 pa, by Avanti Polar Lipids (1 mentions)
Anti gsk3β, by Cell Signaling Technology (1 mentions)
Anti 4e bp1, by Cell Signaling Technology (1 mentions)
Anti ruvbl2, by Proteintech (1 mentions)
Anti fkbp51, by Cell Signaling Technology (1 mentions)
Anti 4ebp2, by Cell Signaling Technology (1 mentions)
Anti tbk1, by Cell Signaling Technology (1 mentions)
Anti eif4e, by Cell Signaling Technology (1 mentions)
Anti mtor, by Fortis Life Sciences (1 mentions)
Anti fkbp12, by Abcam (1 mentions)
Anti sqstm1 p62, by Cell Signaling Technology (1 mentions)
2 protocols using anti raptor
1
Antibody Sources and Reagents for Protein Analysis
2
Antibody-based investigation of mTOR pathway
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The following antibodies were used: anti-mTOR, anti-Raptor, anti-mSIN1, and anti-mLST8 antibodies (Bethyl Laboratories); anti-Frap and anti-DEK antibodies (Santa Cruz Biotechnology); anti-(p-)P70S6KThr389, anti−(p-)AKTThr308, anti−(p-)AKTSer473, anti−(p-)NDRGIThr346, anti−(p-)4E-BP1Thr37/46, anti−(p-)4E-BP1Ser65, anti−(p-)4E-BP1Thr70, anti−(p-)ERK1/2Thr202/Tyr204, anti-p62/SQSTM1, anti-IKBKE, anti−(p-)IKBKESer172, anti-TBK1, anti−(p-)TBK1Ser172, anti−(p-)Tyr, anti−(p-)GSK3-βSer9, anti−GSK3-β, anti-PTK2, anti−(p-)PTK2Tyr398, anti-GRB10, anti−(p-)GRB10Ser501/503, anti−4E-BP1, anti−4E-BP2, anti-eIF4E, and anti−FKBP-51 (Cell Signaling Technologies); anti–p-mTORSer2448 and anti-Rictor antibody (Millipore); anti-LAMP1 and anti–KI-67 (BD Pharmingen); anti–FLAG-M2 and anti-LC3BI/II (Sigma); anti-RUVBL2 (Protein Tech Group); and anti–FKBP-12 (Abcam). ETV7 rabbit polyclonal (15 (link)) was raised in the Grosveld laboratory, and generation of the rat monoclonal antibody (7E4) against a C-terminal peptide (FKDKRPEISP) was outsourced (Absea). Both antibodies were affinity-purified before use. Detection of ETV7 protein immunoprecipitated with 7E4 antibody on most immunoblots was done with a commercial ETV7 antibody (Sigma, #HPA 029033).
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