Cycleavepcr assay

Manufactured by Takara Bio

Sourced in Japan

The CycleavePCR® assay is a real-time PCR-based detection method developed by Takara Bio. It utilizes a unique probe design that allows for the simultaneous amplification and detection of target DNA sequences.

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Lab products found in correlation

Taqman probe based assays, by Thermo Fisher Scientific (1 mentions) Abi prism 310 genetic analyzer, by Thermo Fisher Scientific (1 mentions)

3 protocols using cycleavepcr assay

Genetic Polymorphism Analysis Protocol

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Polymorphisms in EGFR, ABCG2, ABCB1, and POR (Online Resource, Appendix A) were analyzed using TaqMan® probe-based assays (Applied Biosystems, Foster City, CA, USA), whereas the ABCG2 polymorphism (rs2231137) was studied using the CycleavePCR® assay (TaKaRa Bio Inc., Kusatsu, Japan). The detailed genotyping method is provided in the Online Resource (Appendix A).

Ishikawa E., Yokoyama Y., Chishima H., Kasai H., Kuniyoshi O., Kimura M., Hakamata J., Nakada H., Suehiro N., Nakaya N., Nakajima H., Ikemura S., Kawada I., Yasuda H., Terai H., Jibiki A., Kawazoe H., Soejima K., Muramatsu H., Suzuki S, & Nakamura T. (2023). Population Pharmacokinetics, Pharmacogenomics, and Adverse Events of Osimertinib and its Two Active Metabolites, AZ5104 and AZ7550, in Japanese Patients with Advanced Non-small Cell Lung Cancer: a Prospective Observational Study. Investigational New Drugs, 41(1), 122-133.

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Hspa8 T to A Genotyping Protocol

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Genotyping of the T to A substation of the Hspa8 gene was performed by the Amp-FTA method (Nakanishi et al., 2009 ). Templates were prepared on the FTA card and amplified with the following primer set: 5′-ATTAAATATGGGACATTGCTTC-3′ and 5′-CCTTTGTATTCGACTTGGAC-3′. The substitution was detected by Cycleave PCR™ Assay (Takara Bio Inc., Kusatsu, Shiga, Japan), in which fluorescence-labeled DNA–RNA chimeric probes were used. Sequences of the probes were as follows: 5′-ATGGTGG(rA)GA-3′ for the mutant allele and 5′-TGGTGGT(rG)AA-3′ for the WT allele.

Tanaka M., Fujikawa R., Sekiguchi T., Hernandez J., Johnson O.T., Tanaka D., Kumafuji K., Serikawa T., Hoang Trung H., Hattori K., Mashimo T., Kuwamura M., Gestwicki J.E, & Kuramoto T. (2024). A missense mutation in the Hspa8 gene encoding heat shock cognate protein 70 causes neuroaxonal dystrophy in rats. Frontiers in Neuroscience, 18, 1263724.

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Genetic Analysis of Tissue Samples

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Genetic analysis was performed on either the fresh specimens or formalin-fixed paraffin-embedded sections. After nucleic acids were extracted and amplified by polymerase chain reaction, gene mutations were analyzed by ABI PRISM 310 Genetic Analyzer (Applied Biosystems) or the Cycleave PCR assay (Takara Co., Ltd); the detail of which was described previously [16 (link), 17 (link)].

Hijioka S., Hosoda W., Mizuno N., Hara K., Imaoka H., Bhatia V., Mekky M.A., Tajika M., Tanaka T., Ishihara M., Yogi T., Tsutumi H., Fujiyoshi T., Sato T., Hieda N., Yoshida T., Okuno N., Shimizu Y., Yatabe Y., Niwa Y, & Yamao K. (2014). Does the WHO 2010 classification of pancreatic neuroendocrine neoplasms accurately characterize pancreatic neuroendocrine carcinomas?. Journal of Gastroenterology, 50(5), 564-572.

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