7100 automatic biochemical analyzer

Manufactured by Hitachi

Sourced in Japan

The 7100 automatic biochemical analyzer is a laboratory equipment designed to perform automated biochemical analyses. It is capable of performing a variety of tests on biological samples to measure the levels of different chemical substances.

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Close spelling variants of this product

Automatic biochemical analyzer (128 citations) Automatic analyzer (51 citations) Biochemical analyzer (14 citations) 7100 Automatic Analyzer (5 citations) 7100 blood automatic biochemical analyzer (2 citations)

13 protocols using 7100 automatic biochemical analyzer

Urinary NAG and Blood BUN/Scr Analysis

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The levels of NAG (cat no. A031) in the urine were determined using the nitrophenol colorimetric method, and the levels of BUN (cat. no. C013-2) and Scr (cat. no. C011-1) in the peripheral blood were detected using a 7100 automatic biochemical analyzer (Hitachi, Ltd.) using specific kits purchased from Nanjing Jiancheng Bioengineering Research Institute. All procedures were performed strictly in accordance with the manufacturer's protocol (Nanjing Jiancheng Bioengineering Institute).

Liang T., Wei C., Lu S., Qin M., Qin G., Zhang Y., Zhong X., Zou X, & Yang Y. (2021). Ginaton injection alleviates cisplatin-induced renal interstitial fibrosis in rats via inhibition of apoptosis through regulation of the p38MAPK/TGF-β1 and p38MAPK/HIF-1α pathways. Biomedical Reports, 14(4), 38.

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Biomarker Analysis in Kidney Function

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BUN (cat. no. C013-2), Scr (cat. no. C011-1) and urinary NAG (cat. no. A031) levels were determined using the corresponding assay kits obtained from Nanjing Jiancheng Bioengineering Institute according to the manufacturer’s instructions. The levels of BUN and Scr were measured with a 7100 automatic biochemical analyzer (Hitachi, Ltd.). The level of urinary NAG was detected using the nitrophenol colorimetric method with a continuous spectrum scanning microplate reader (Spectra Maxplus 384; Molecular Devices, LLC) at a wavelength of 400 nm.

Wei C., Zhang Y., Zhong X., Lu S., Zou X., Yang Y., Huang S, & Huang Z. (2022). Ginkgobiloba leaf extract mitigates cisplatin-induced chronic renal interstitial fibrosis by inhibiting the epithelial-mesenchymal transition of renal tubular epithelial cells mediated by the Smad3/TGF-β1 and Smad3/p38 MAPK pathways. Chinese Medicine, 17, 25.

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Serum Fractionation using FPLC

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Mice serum was fractionated using FPLC (Akta FPLC; GE Healthcare, Pittsburgh, PA, USA) using Superose 6 Increase 10/300 GL columns. Phosphate buffered saline (PBS; pH 7.4) eluent buffer was first passed through a 0.22 μm filter, and then the column was equilibrated at a rate of 0.25 mL/min. Serum sample aliquots of 500 μL were then injected into the column. Elutions were then carried out at a flow rate of 0.25 mL/min. 63 fractions, 0.3 mL each, were collected in separate eluant eppendorf tubes for subsequent cholesterol content analysis by 7100 automatic biochemical analyzer (HITACHI).

Wang X., Chen X., Zhang X., Su C., Yang M., He W., Du Y., Si S., Wang L, & Hong B. (2020). A small-molecule inhibitor of PCSK9 transcription ameliorates atherosclerosis through the modulation of FoxO1/3 and HNF1α. EBioMedicine, 52, 102650.

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Endotoxin-Induced Organ Dysfunction Study

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LPS (Lipopolysaccharides from Escherichia coli O555: B5, L2880-100MG) was purchased from Sigma (USA) (Batch number: 017M4112V). Dexamethasone (DXM) was purchased from Anhui Golden Sun Biochemical Pharmaceutical Co., Ltd. (Anhui, China) (batch number: 15032521). 7100 Automatic biochemical analyzer (Hitachi, Japan), Bio-Rad electrophoresis unit (USA), and Bio-Rad ChemiDocXRS + Gel Imaging System (USA) were used in this study. LEICA RM2235 paraffin slicer (Germany) was used to generate sections for microscopy. LEICA DM2500 Optical Microscope (Germany) was used to measure neutrophil invasion. Urea nitrogen (BUN) (R1 TG836, R2 TG837), total protein (TP) (TH619), albumin (ALB) (TF126), aspartate aminotransferase (AST) (R1 AR792, R2 TG862), alanine aminotransferase (ALT) (R1 AR794, R2 TH622), lactate dehydrogenase (LDH) (R1 AR796, R2AP318) and alkaline phosphatase (ALP) (R1 TF168, R2 AR800) were all purchased from Japan Pure Pharmaceutical Industry Co., Ltd. in Shanghai, China. Creatinine (Cre) (710241 H) and creatine kinase (CK) (708021 G) were purchased from Beijing Leadman Biochemical Co., Ltd. (Beijing, China).

Gong Q., He L., Wang M., Zuo S., Gao H., Feng Y., Du L., Luo Y, & Li J. (2019). Comparison of the TLR4/NFκB and NLRP3 signalling pathways in major organs of the mouse after intravenous injection of lipopolysaccharide. Pharmaceutical Biology, 57(1), 555-563.

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Serum and Liver Lipid Profile Analysis

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After slowly dissolving the serum samples from each treatment group and storing them in a refrigerator at −80 °C and at 4 °C, the sera were sequentially placed into a Hitachi 7100 automatic biochemical analyzer for detection. The contents of TG, TC, LDL-C, and HDL-C and the activity of ALP in the sera were, respectively, obtained.
The insulin doses were determined according to the instructions of the mouse insulin ELISA kit. The insulin resistance level was calculated according to the following Formula (1).

HOMA-IR = \frac{C_{1} \times C_{2}}{22.5}

where C₁ is the blood glucose concentration, mmol/L; C₂ is the blood insulin concentration, mU/L.
The collected blood was centrifuged at 600 g for 10 min to obtain the supernatant. After diluting it to a certain proportion, the activity of SOD and the contents of MDA and GSH in plasma were measured according to the instructions of the kit.
Part of the weighed liver tissue was homogenized by adding 0.2–0.3 mL of PBS in an ice water bath, and the homogenate was taken to detect TC and TG in the liver tissue according to the instructions of the kits.

Geng N., Li Y., Zhang Y., Wang H., Song J., Yu L, & Wu C. (2023). Effects of Modified Dietary Fiber from Fresh Corn Bracts on Obesity and Intestinal Microbiota in High-Fat-Diet Mice. Molecules, 28(13), 4949.

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Rat Urine Analysis for Kidney Function

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The urine of the rats was collected using ZH-B6 metabolite cages (Anhui Zhenghua Biological Instrument Equipment Co., Ltd., Anhui, China). Blood and urine samples were collected for biochemical analysis, the kidneys and livers of rats were isolated and stored at −80 °C for expression analysis.
The quantity of urine collected after 24 h was determined, and the protein content of the urine was measured. Serum was isolated from rat blood for biochemical tests. Serum BUN (sBUN), serum Cre (sCre), sALB, and sTP were analyzed using a 7100 automatic biochemical analyzer (Hitachi, Japan), and urine Cre (uCre), mALB, and CSF were analyzed in the same manner as the serum index (Gong, He, Wang, Zou, et al. 2019 (link)). uTP = urine volume × CSF. uALB = urine volume × mALB. Cre clearance rate = (uCre × urine volume)/(sCre × 1440) (National Kidney Foundation 2002 (link); Levey et al. 2020 (link)).

Gong Q., Yin J., Wang M., He L., Lei F., Luo Y., Yang S., Feng Y., Li J, & Du L. (2020). Comprehensive study of dexamethasone on albumin biogenesis during normal and pathological renal conditions. Pharmaceutical Biology, 58(1), 1261-1271.

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Serum Biochemical Markers of Liver Damage

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The blood was centrifuged at 3000× g for 10 min to separate serum. Liver damage was assessed by the estimation of serum activities of ALT and AST, using commercially available test kits provided by Nanjing Jiancheng Bioengineering Institute. In addition, the serum levels of LDH and TG were estimated by Hitachi 7100 automatic biochemical analyzer.

Huang Z.Q., Chen P., Su W.W., Wang Y.G., Wu H., Peng W, & Li P.B. (2018). Antioxidant Activity and Hepatoprotective Potential of Quercetin 7-Rhamnoside In Vitro and In Vivo. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(5), 1188.

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Alzheimer's Disease Lipid Biomarkers

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The protocol of this study was approved by the Ethics Committee of Guangzhou General Hospital of Guangzhou Military Command. Informed consent was obtained from all participants.
This case–control study enrolled 207 patients diagnosed with AD and 256 healthy control subjects in the Home for the Aged Guangzhou, Guangzhou Brain Hospital, and Guangzhou General Hospital of Guangzhou Military Command. All participants were 65 years of age or older, unrelated and from the Chinese Han population. All AD cases were diagnosed as “definite” or “probable” by the National Institute of Neurological and Communicative Disorders and Stroke/Alzheimer Disease and Related Disorders Association. Patients with vascular dementia, dementia caused by systemic diseases or poisoning, depressive pseudo dementia, and/or advanced, severe, progressive, or unstable infectious, metabolic, or immunologic diseases were excluded. Blood glucose (GLU), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured using a 7100 automatic biochemical analyzer (Hitachi, Ltd. Tokyo, Japan).

Zheng J., Yan H., Shi L., Kong Y., Zhao Y., Xie L., Li J., Huang M., Li J, & Zhao S. (2016). The CYP19A1 rs3751592 variant confers susceptibility to Alzheimer disease in the Chinese Han population. Medicine, 95(35), e4742.

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Comprehensive Metabolic Profiling Protocol

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The blood glucose was determined with blood glucose test strips
Urine and serum were collected as previously described [12 (link)] for the detection of urinary protein and liver and kidney function. Overnight urine was collected, the urine volume was accurately measured, the total urine protein (uCSF) and urine microalbumin (umALB) were detected by a 7100 automatic biochemical analyzer (Hitachi, Japan), and finally, by multiplying by urine volume, total urinary protein and microalbumin were obtained. The liver and kidney function was evaluated by the index of ALT, AST, BUN and CRE. All the samples in serum were detected according to the kid instructions by using the 7100 automatic biochemical analyzer.

Wang M., Gong Q., Zha C., Xu S., Yu D., Huang T., Feng Y., Sun H, & Li J. (2023). Low-dose lipopolysaccharide inducing continuous and obvious increase in urinary protein in hyperglycemic rats and the underlying mechanism. PLOS ONE, 18(7), e0288876.

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Plasma TG Extraction and Analysis

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Blood was collected from the orbital sinus into sterile 1.5 ml tubes containing citrate sodium (3 M). Then, blood cells were removed by centrifugation at 2,000 g for 20 min at 4°C, the supernatant was immediately aliquoted and stored at -80°C. Plasma was prepared for TG analysis with 7100 automatic biochemical analyzer (Hitachi). Hepatic TG was performed according to the protocol of the TG assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1)

Huang K., Zhang T., Zhang W., Gu Y., Yu P., Sun L., Liu Z., Wang T, & Xu Y. (2023). Multigenerational mistimed feeding drives circadian reprogramming with an impaired unfolded protein response. Frontiers in Endocrinology, 14, 1157165.

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