Hrp conjugated anti rat igg na935v

Total cell lysates were collected with CelLytic MT Cell Lysis Reagent (Sigma), followed by one-dimensional SDS-PAGE, and were electrophoretically transferred onto a piece of Immobilon (Millipore, Burlington, MA, USA). The membrane was saturated with PBS containing 4% skimmed milk and treated with primary antibodies. Supernatants of rat anti-CLDN12 hybridoma were directly used as primary antibodies, whereas rabbit anti-CLDN12 pAb was diluted at 1:2000 in PBS. The signal was detected by chemiluminescence using 1:2000-times diluted HRP-conjugated anti-rat IgG (NA935V, GE Health Care, Chicago, IL, USA) or anti-rabbit IgG (NA934V, GE Health Care).

Total cell lysates were collected with CelLytic MT Cell Lysis Reagent (Sigma) followed by one-dimensional SDS-PAGE and electrophoretically transferred onto a piece of Immobilon (Millipore). The membrane was saturated with PBS containing 4% skimmed milk and treated with primary antibodies. For screening of anti-CLDN6 rat mAbs, supernatants of hybridoma were directly used as primary antibodies, whereas anti-CLDN6 rabbit pAb was diluted at 1:2000 in PBS. The signal was detected by chemiluminescence using HRP-conjugated anti-rat IgG (NA935V, GE Health Care, Chicago, IL, USA) or anti-rabbit IgG (NA934V, GE Health Care).