Ab45139

Antibodies against PAI-1 (ab222754, 1:500; ab125687, 1:100), anti-VTN (ab45139, 1:2000), anti-LRP1 (ab168454, WB 1:1000, IP 1:100), anti-SMAD2 (phosphor S467) (ab280888), anti-SMAD2(ab40855), anti-SMAD3 (phosphor S423+S425) (ab172202), anti-SMAD3 (AB40854) were purchased from Abcam. Anti-PLAT (sc-515562, 1:50) and anti-PLAU (sc-59727, 1:500) were purchased from Santa cruz. Anti- Phospho-STAT1 (Tyr701) (7649T), anti-STAT1 (14994T), anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/tyr204) (4370), anti p44/42 MAPK (Erk1/2) (4695), anti-Phospho-Akt (Ser473) (4060), anti-Akt (14702) anti-phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (4228), anti-PI3 Kinase p85(4257), anti-E-Cadherin (3195T), anti-N-Cadherin (13116T), anti-phospho- NF-κB p65 (Ser536) (3033s), anti- NF-κB p65 (8242) were purchased from CST. Anti-Anti-GAPDH (10494-1-AP) was purchased from Proteintech. His tagged human PAI-1 (10296-H08H) was purchased from Sino Biological. His-PLAU (PLU-H5229) was purchased from Acro. Human Lys-plasminogen (HPG2002), Glu-plasminogen (HPG2001) and S-2251 (100-01) were purchased from Enzyme Research Laboratories. Z-GGR-AMC was purchased from A+ peptide (shanghai). Recombinant Human LRP1-Cluster II-Fc Chimera protein (2368-L2) was purchased from Bio-Techne.

For histological analysis, the following primary antibodies (Abs) were employed: anti-vitronectin Abs (ab45139, Abcam, Cambridge, MA, and MAB38751, R&D Systems, Inc., Minneapolis, MN), anti-thrombospondin 1 Abs (Ab-11, Thermo Fisher Bio-scientific, Hudson, NH, and ab1823, Abcam), anti-CD45 Abs (ab10558, Abcam, and M0701, DAKO, Carpinteria, CA, USA). Additionally, for B220⁺CD11c⁺NK1.1⁺ NK cell sorting, the following antibodies were used; PE/Cy7 anti-mouse B220 (103222, BioLegends), PE anti-mouse CD11c (117308, BioLegends), APC anti-mouse NK1.1 (108710, BioLegends), and those isotype control antibodies, PE/Cy7 Rat IgG2a, κ (400521, BioLegends), PE American Hamster IgG (12-4888-81, Thermo Fisher), APC Mouse IgG2a κ (550882, BD Biosciences).