Uv 1601 pc

Manufactured by Shimadzu

Sourced in Japan

The UV-1601 PC is a UV-Visible spectrophotometer designed for general laboratory use. It features a wavelength range of 190 to 1100 nanometers and can be used to measure the absorbance, transmittance, or reflectance of liquid, solid, or gas samples.

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Spelling variants of this product

UV-1601 (459 citations) UV-1601 PC spectrophotometer (45 citations) Model UV-1601 PC (18 citations) UV-Vis 1601 PC (8 citations) 1601 PC UV-visible Spectrophotometer (5 citations)

176 protocols using uv 1601 pc

Quantification of Total Drug and Free Drug in FLZ-NLCs

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For total drug content in FLZ-NLCs formulation, 0.2 mL of the formulation and placebo NLCs were taken in two different microcentrifuge tubes and diluted with 0.8 mL of methanol which was centrifuged using cooling centrifuge (Sigma Laborzentrifugen GmbH, 3K30, Germany) at 54,200 g for 30 min at 4 °C. The supernatant was collected and filtered using a syringe filter (0.22 µ).
The absorbance of filtrate was measured using placebo NLCs formulation as a blank at 261 nm after 10 times dilution by using UV-Spectrophotometer (UV-1601PC, Shimadzu Corporation, Kyoto). The drug content (DC) of FLZ-NLCs was calculated using the formula given below:
For presence of free drug in FLZ-NLCs formulation, 0.5 mL of the formulation and placebo NLCs were taken in two different microcentrifuge tubes and diluted with 0.5 mL of milli Q water which was centrifuged using cooling centrifuge (Sigma Laborzentrifugen GmbH, 3K30, Germany) at 54,200 g for 30 min at 4 °C. The supernatant was collected and filtered using a syringe filter (0.22 µ). The absorbance of filtrate was measured at 261 nm by using UV -Spectrophotometer (UV-1601PC, Shimadzu Corporation, Kyoto) (Pardeike et al., 2011) . The entrapment efficiency (EE) of FLZ-NLCs was calculated using the formula:

Fernandes A.V., Pydi C.R., Verma R., Jose J., & Kumar L. (2020). Design, preparation and in vitro characterizations of fluconazole loaded nanostructured lipid carriers. Brazilian Journal of Pharmaceutical Sciences, 56.

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Spectrophotometric and HPLC Analysis

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A Shimadzu (Kyoto, Japan) UV-1601 PC, UV–visible double-beam spectrophotometer was used. The third derivative spectra of the drugs were derived in the wavelength range (200–400) nm using Δλ = 8 nm and scaling factor = 10.
A Shimadzu LC-20 AD prominence liquid chromatograph (Japan) was used for HPLC analysis; with a Rheodyne injector valve and a SPD-20A UV detector set at wave length 215 nm.

Aly F.A., EL-Enany N., Elmansi H, & Nabil A. (2017). Simultaneous determination of cetirizine, phenyl propanolamine and nimesulide using third derivative spectrophotometry and high performance liquid chromatography in pharmaceutical preparations. Chemistry Central Journal, 11, 99.

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In Vitro Temazepam Release from NLC Formulation

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The dialysis membrane diffusion technique [32 (link),33 (link),34 (link)] was applied to analyze the in vitro release of optimized temazepam NLC formulation (NLC-1) compared with temazepam suspension. PBS (pH 7.4, 40 mL) was selected as the release vehicle. An accurately weighed amount of freeze-dried temazepam-loaded NLC-1 dispersion in PBS, pH 7.4, equivalent to (141 µg/mL) temazepam was added to a glass cylinder fitted at its lower end with presoaked cellulose membrane (Spectra/Por^® dialysis membrane, M.W. cut off 12,000–14,000). The glass cylinder was then immersed in a beaker containing the release medium at 37 ± 0.5 °C and agitated at a constant speed of 100 rpm. Aliquots (3 mL) were withdrawn and substituted with fresh medium. The drug content was determined spectrophotometrically using Shimadzu, model UV-1601 PC (Kyoto, Japan), λ = 232 nm, at predetermined time intervals for 24 h. The release experiment was performed in triplicate. Various mathematical models were applied, in order to determine the kinetics and mechanism of drug release from the developed NLC formulation.

E. Eleraky N., M. Omar M., A. Mahmoud H, & A. Abou-Taleb H. (2020). Nanostructured Lipid Carriers to Mediate Brain Delivery of Temazepam: Design and In Vivo Study. Pharmaceutics, 12(5), 451.

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Quantifying Total Phenolic Content in Ginger and Garlic

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The total phenolic content (TPC) of ginger and garlic extracts was determined using the Folin-Ciocalteu method, according to previously described procedures [31 (link)]. Precisely, 10 mg of gallic acid was dissolved in 100 ml of 50% methanol and diluted to 10, 20, 30, 40, 50, and 60 g/ml. An aliquot (1 ml) from each dilution was transferred to a test tube and diluted with 10 ml of distilled water. Then 2 ml of Folin-Ciocalteu's reagent was added, vortexed, and left to incubate for 5 min at room temperature. In each test tube, 4 ml of 0.7 M Na₂CO₃ were added, adjusted with distilled water up to the mark of 25 ml, vortexed, and held for 30 min at room temperature. The absorbance of the standard was measured against a blank using a UV/VIS spectrophotometer (Shimadzu model UV-1601 PC, Kyoto, Japan) (distilled water). Similarly, 1 ml of the sample extracts were prepared and analyzed using a comparable process. A standard curve was then plotted using garlic acid, and total phenolic contents were calculated using the linear equation on the standard curve. Total phenolic components were calculated as mg/100 g extract of gallic acid equivalent (GAE).

Akullo J.O., Kiage-Mokua B.N., Nakimbugwe D., Ng’ang’a J, & Kinyuru J. (2023). Phytochemical profile and antioxidant activity of various solvent extracts of two varieties of ginger and garlic. Heliyon, 9(8), e18806.

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Spectrophotometric Determination of Drug Content

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To determine the drug content, 0.1 ml of the dispersion was appropriately diluted using methanol. Afterward, the UV absorbance of the diluted sample was determined using a UV spectrophotometer (model UV-1601 PC, Shimadzu, Kyoto, Japan) at λ_max 244 nm (Dong et al., 2018 (link)). The drug content was measured using the following equation (El-Leithy et al., 2018 (link)):

Drug content (%) = \frac{Actual yield}{Theoritical yield} x 100

Elgendy H.A., Makky A.M., Elakkad Y.E., Ismail R.M, & Younes N.F. (2023). Syringeable atorvastatin loaded eugenol enriched PEGylated cubosomes in-situ gel for the intra-pocket treatment of periodontitis: statistical optimization and clinical assessment. Drug Delivery, 30(1), 2162159.

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Serum Lipid and Uric Acid Analysis

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Serum total cholesterol, triglycerides and uric acid were determined using colorimetric kits (Biodignostic, Cairo, Egypt) using a UV-visible spectrophotometer (UV-1601PC, Shimadzu, Japan) following the manufacturer's instructions.

Mahmoud A.A, & Elshazly S.M. (2014). Ursodeoxycholic Acid Ameliorates Fructose-Induced Metabolic Syndrome in Rats. PLoS ONE, 9(9), e106993.

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Characterization of MTX-Loaded ZnO Nanoparticles

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MTX-ZnONPs were characterized by UV-vis spectrophotometry (Shimadzu dual-beam spectrophotometer, model UV-1601 PC, 1 nm resolution. Kyoto, Japan). The mean particle size and zeta potential of MTX-ZnONPs were determined using a particle size analyzer (Zetasizer Nano-ZS, Model ZEN3600, Malvern Instruments Ltd., Malvern, United Kingdom) [(Gomathy and Sabarinathan, 2010 ), 15]. Transmission electron microscope (Tecnai™ G2 Spirit Bio-TWIN, FEI, Hillsboro, United States) was used to investigate the size of the inorganic core at a accelerating voltage of 80 kV (Khan S. et al., 2018 (link)). The binding confirmation of MTX on the surface of ZnONPs was analyzed by Fourier-transform infrared spectroscopy (FTIR) (Shimadzu FTIR-8201 PC infrared spectrophotometer) operating in the diffuse reflectance mode at a resolution of 4 cm⁻¹. A total of 256 scans of the ZnONPs film (400–4,000 cm⁻¹ range) were acquired to obtain good signal-to-noise ratios.

Mishra P., Ali Ahmad M.F., Al-Keridis L.A., Saeed M., Alshammari N., Alabdallah N.M., Tiwari R.K., Ahmad A., Verma M., Fatima S, & Ansari I.A. (2023). Methotrexate-conjugated zinc oxide nanoparticles exert a substantially improved cytotoxic effect on lung cancer cells by inducing apoptosis. Frontiers in Pharmacology, 14, 1194578.

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Spectrophotometric Analysis Using Shimadzu UV-1601

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A Shimadzu UV-1601 PC (Kyoto, Japan), UV–Visible double-beam spectrophotometer was used to perform spectrophotometric analysis with matched 1 cm path-length quartz cells and 1 nm slit width.

Elama H.S., Zeid A.M., Shalan S.M., El-Shabrawy Y, & Eid M.I. (2022). Eco-friendly spectrophotometric methods for determination of remdesivir and favipiravir; the recently approved antivirals for COVID-19 treatment. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 287, 122070.

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Determining Encapsulation Efficiency of Liposome-Xanthone Formulations

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The %EE of LX was calculated indirectly by measuring the free LX (nonencapsulated LX) in the dispersion medium. One milliliter of dispersion medium was subjected to centrifugation via a cooling centrifuge (3K30, Sigma, Germany) at 15,000 rpm for one hour at 4°C. The supernatant was detached, diluted with ethanol (95%). The concentration of free LX was spectrophotometrically calculated (Shimadzu, model UV-1601 PC, Kyoto, Japan) at λmax 381 nm via the calibration curve (n=3, R²=0.9999). Each formula was tested three times. The %EE was computed using the subsequent formula:21–23 (link)

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$$\eqalign{& \% {\rm{EE = }}{{\left( {{\rm{total}}\;{\rm{amount}}\;{\rm{of}}\;{\rm{LX - total}}\;{\rm{amount}}\;{\rm{of}}\;{\rm{free}}\;{\rm{LX}}} \right)} \over {{\rm{total}}\;{\rm{amount}}\;{\rm{of}}\;{\rm{LX}}}} \cr & \,\,\,\,\,\,\,\,\,\,\,\,\,\,\,\,{\rm{X100}} \cr} $$
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Total amount of LX is the actual weighed amount (8 mg), total amount of free LX (amount of LX in supernatant)

Ahmed S., Kassem M.A, & Sayed S. (2020). Bilosomes as Promising Nanovesicular Carriers for Improved Transdermal Delivery: Construction, in vitro Optimization, ex vivo Permeation and in vivo Evaluation. International Journal of Nanomedicine, 15, 9783-9798.

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Mucin Interaction with Optimized STZ-MMs

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Each of optimized STZ-MMs were mixed with 0.1% w/w aqueous mucin solution (1:40) and incubated at 37 ± 2 °C for 24 h at 100 rpm. At three different time points (6, 12, and 24 h), the PS and ZP of the dispersions were measured as mentioned previously. Also, the turbidity of the incubated dispersions was evaluated by measuring the transmittance % (T%) spectrophotometrically at λ_max 500 nm (Shimadzu UV-1601PC, Kyoto, Japan) (Di Prima et al., 2017 (link)). The turbidity was calculated according to the following equation (Abdelbary and Tadros, 2013 (link)):

Turbidity = \frac{(100 - T %)}{100}

Results were compared with ZP, PS, and turbidity values measured for mucin solution and aqueous MMs dispersions.

Younes N.F., Abdel-Halim S.A, & Elassasy A.I. (2018). Solutol HS15 based binary mixed micelles with penetration enhancers for augmented corneal delivery of sertaconazole nitrate: optimization, in vitro, ex vivo and in vivo characterization. Drug Delivery, 25(1), 1706-1717.

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