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Prep kit v2

Manufactured by Illumina
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The Prep Kit V2 is a laboratory equipment product offered by Illumina. It is designed to prepare samples for use with Illumina's sequencing platforms. The core function of the Prep Kit V2 is to enable the preparation of DNA or RNA samples for sequencing, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Nextseq sequencer, by Illumina (1 mentions)

Spelling variants of this product

TruSeq RNA Sample Prep Kits v2 (27 citations) TrueSeq RNA Library Prep Kit v2 (12 citations) TruSeq Stranded mRNA Sample Prep Kit v2 (10 citations) TruSeq RNA Sample Prep Kit v2 protocol (7 citations) V2 kits (4 citations) TruSeq Prep Kit v2 (4 citations) TruSeq RNA Sample Prep Kit v2 kit (4 citations) TruSeq® Small RNA Library Prep Kits v2 (3 citations) Kit v2 (2 citations) VAHTS Universal DNA Library Prep Kit for Illumina V2 (2 citations)

2 protocols using prep kit v2

1

Optimized RNA-seq Library Preparation

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After the extraction of total RNA, the sequencing library was generated using the Prep Kit V2 (Illumina, San Diego, CA, USA) for RNA Library according to the manufacturer’s procedure. RNA-seq library was sequenced using the 75-nucleotide paired-end sequencing protocol on a NextSeq sequencer (Illumina). The technical replicates are duplicates for each sample and the RNAseq details are as follows: sequencing depth: 34.10939x; dispersions: 0.017797; effect 2; false positive rate: 0.05. The statistical power of this experimental design, calculated in RNASeqPower is 0.98. For bioinformatics analysis: genes whose Fold Change (padj <= 0.05) is less than or equal to -2 were defined as down-regulated genes (CHRAC1 knockdown versus control cell). Heat-map for different cancer hallmarks was created by TB-tools. The sequencing data have been deposited in the FigShare (DOI: 10.6084/m9.figshare.23989101).
Li S., Wang L., Shi J., Chen Y., Xiao A., Huo B., Tian W., Zhang S., Yang G., Gong W, & Zhang H. (2024). Chromatin accessibility complex subunit 1 enhances tumor growth by regulating the oncogenic transcription of YAP in breast and cervical cancer. PeerJ, 12, e16752.
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2

RNA Extraction and Analysis from FFPE Samples

Cited 1 time
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The methods used to extract, sequence, and analyze RNA from FFPE samples to generate digital gene expression values were followed as described previously (16 (link)). An RNA library employing TruSeq (Illumina Prep Kit v2) was prepared and genes with zero signal on more than 20% of the samples were excluded from analysis.
Pittaluga S., Nicolae A., Wright G.W., Melani C., Roschewski M., Steinberg S., Huang D., Staudt L.M., Jaffe E.S, & Wilson W.H. (2020). Gene Expression Profiling of Mediastinal Gray Zone Lymphoma and Its Relationship to Primary Mediastinal B-cell Lymphoma and Classical Hodgkin Lymphoma. Blood Cancer Discovery, 1(2), 155-161.
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