Fitc conjugated cd45
FITC-conjugated CD45 is a fluorescently-labeled antibody that targets the CD45 cell surface antigen. CD45 is a tyrosine phosphatase expressed on the surface of most hematopoietic cells. The FITC (Fluorescein Isothiocyanate) fluorophore is conjugated to the CD45 antibody, allowing for the detection and identification of CD45-positive cells using flow cytometry.
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3 protocols using fitc conjugated cd45
Flow Cytometry Analysis of Mesenchymal Stem Cell Markers and Extracellular Vesicles
Isolation and Osteogenic Differentiation of BMSCs
After detecting the purity, BMSCs from the second passage were used in subsequent experiments. To induce osteogenic differentiation, BMSCs were initially cultured in complete culture media as aforementioned. When the cells reached 80% confluence, osteogenic-inducing medium [α-MEM (Cyagen Biosciences) supplemented with 10% FBS, 50 mg/ml ascorbate, 10 mM β-glycerophosphate, 100 nM dexamethasone and 1% penicillin-streptomycin] was used. Lastly, the plates were stained with alizarin red and alkaline phosphatase at four time-points (0, 3, 7, and 14 days).
Immunophenotyping of Mesenchymal Stem Cells
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