ChiP analysis was performed as previously described.10 (link) In brief, K562 cells were cultured in T25 flasks with approximately 15 million cells in 10ml of complete growth medium containing DMSO, 10µM, or 20µM ICG-001 for 24 hours prior to cross-linking with 1% formaldehyde. Cells were then lysed and sonicated. Normal IgG (Santa Cruz, sc-2027), anti-CBP ( A22, Santa Cruz, sc-369) or anti-p300 (N15, Santa Cruz, sc-584) were then added to the sheared chromatin. After 24 hours, agarose beads were added and DNA was eluted from beads. 1/10 of the elution was used for qPCR analysis on a BioRad MyIQ real time PCR detection system. The occupancy of CBP or p300 at the survivin promoter was determined by the Fold Enrichment Method (ThermoFisher Science, ChiP analysis). The primers used in the study were: forward: hu-Survivin ChIP-F 5’-CTC CAG GAC TCA AGT GAT GC-3’; and reverse hu-Survivin ChIP-R 5’-CCG CGG CCT TCT GGG AGT AG-3’
Anti cbp a22
Manufactured by Santa Cruz Biotechnology
Anti-CBP (A22) is a laboratory reagent used for research purposes. It functions as an antibody that specifically binds to the CREB-binding protein (CBP), a transcriptional co-activator involved in various cellular processes. This product can be utilized in techniques such as immunoprecipitation, Western blotting, and other applications where the detection or isolation of CBP is required.
Automatically generated - may contain errors
Lab products found in correlation
Normal igg, by Santa Cruz Biotechnology (2 mentions)
Anti p300, by Santa Cruz Biotechnology (2 mentions)
Anti gfp b 2, by Santa Cruz Biotechnology (1 mentions)
Anti myc 4a6, by Merck Group (1 mentions)
Anti phospho smad2 ser465 467 138d4, by Cell Signaling Technology (1 mentions)
Rabbit igg, by Southern Biotech (1 mentions)
Anti acetyl histone h3, by Merck Group (1 mentions)
Anti ha y 11, by Santa Cruz Biotechnology (1 mentions)
Anti β actin ac 15, by Merck Group (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
3 protocols using anti cbp a22
1
ChIP Analysis of Survivin Promoter
2
Comprehensive Antibody Characterization Protocol
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The following commercially available antibodies used were: anti-PAI-1 (clone 41/PAI-1; BD Biosciences), anti-p53 (DO-1; Calbiochem), anti-β-actin (AC-15; Sigma), anti-phospho-Smad2 (Ser465/467) (138D4; Cell Signaling Technology), anti-Smad2/3 (clone 18/Smad2/3; BD Bioscience), anti-CBP (A-22; Santa Cruz Biotechnology), anti-acetyl-Histone H3 (catalog no. 06–599; EMD Millipore), anti-Myc (4A6; EMD Millipore), anti-FLAG (M2; Sigma), anti-HA (Y-11; Santa Cruz Biotechnology), and anti-GFP (B-2; Santa Cruz Biotechnology). Mouse immunoglobulin G1 (IgG1) (MB002; R & D Systems) and rabbit IgG (Southern Biotech) were used as controls.
3
ChIP Analysis of Survivin Promoter
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChiP analysis was performed as previously described.10 (link) In brief, K562 cells were cultured in T25 flasks with approximately 15 million cells in 10ml of complete growth medium containing DMSO, 10µM, or 20µM ICG-001 for 24 hours prior to cross-linking with 1% formaldehyde. Cells were then lysed and sonicated. Normal IgG (Santa Cruz, sc-2027), anti-CBP ( A22, Santa Cruz, sc-369) or anti-p300 (N15, Santa Cruz, sc-584) were then added to the sheared chromatin. After 24 hours, agarose beads were added and DNA was eluted from beads. 1/10 of the elution was used for qPCR analysis on a BioRad MyIQ real time PCR detection system. The occupancy of CBP or p300 at the survivin promoter was determined by the Fold Enrichment Method (ThermoFisher Science, ChiP analysis). The primers used in the study were: forward: hu-Survivin ChIP-F 5’-CTC CAG GAC TCA AGT GAT GC-3’; and reverse hu-Survivin ChIP-R 5’-CCG CGG CCT TCT GGG AGT AG-3’
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