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Rnascope multiplex assay kit

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The RNAscope Multiplex Assay Kit is a tool for the detection and visualization of multiple RNA targets within a single tissue sample. It enables the simultaneous analysis of up to 4 different RNA transcripts in a single experiment, providing a comprehensive view of gene expression patterns.

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RNAscope kit Multiplex assay Multiplex kits

2 protocols using rnascope multiplex assay kit

1

RNA Extraction and FISH Assay

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The RNA in the cytoplasm and nucleus was extracted by using Nuclear/cytoplasmic fractionation PARIS Kit (Life Technologies, CA, USA) following manufacturer’s recommendations. GAPDH and U6 were used as cytoplasmic and nucleus reference fragments, respectively. FISH assay was carried out by using RNAscope Multiplex Assay Kit (Thermo Fisher Scientific, MA, USA) according to manufacturer’s instructions, the fluorescence signal was then observed with microscopy.
Liu J., Liu H., Zeng Q., Xu P., Liu M, & Yang N. (2020). Circular RNA circ-MAT2B facilitates glycolysis and growth of gastric cancer through regulating the miR-515-5p/HIF-1α axis. Cancer Cell International, 20, 171.
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2

Subcellular Localization of HOTAIR lncRNA

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The subcellular localization of lncRNA HOTAIR in NP cells was determined with firm compliance to the instructions of the RNAscope Multiplex Assay kit (Thermo Fisher). The HOTAIR fluorescent probe was designed and provided by the kit's manufacturer [23 ].
Zhang S., Song S., Cui W., Liu X, & Sun Z. (2022). Mechanism of Long Noncoding RNA HOTAIR in Nucleus Pulposus Cell Autophagy and Apoptosis in Intervertebral Disc Degeneration. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 8504601.
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