Cell culture supernatants from the 48-well plates (as described in the cell activation section) were collected after five days of stimulation, and both IL-10 and IFNγ secretion was determined by ELISA specific for human IL-10 (BD Pharmingen, San Diego, CA) and IFNγ (Endogen, Rockford, IL). Alternatively, cytokine secretion was also determined using the IL-10 and IFNγ secretion assays from Miltenyi using anti-IFNγ-FITC and anti-IL-10-PE detection reagents, according to the manufacturer’s instructions and cells were gated on CD3-APC+ T cells. Gates were done using the unstimulated T cells and were also verified using unstained activated T cells.
Elisa specific for human il 10
Manufactured by BD
The ELISA specific for human IL-10 is a laboratory tool used to quantify the levels of the human cytokine interleukin-10 (IL-10) in samples. The assay employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of IL-10 in various biological matrices.
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Lab products found in correlation
2 protocols using elisa specific for human il 10
1
Cytokine Production Assessment in T Cells
2
Cytokine Production Assessment in T Cells
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