Tissue lysates were subjected to SDS-polyacrylamide gel electrophoresis, and transferred onto PVDF membranes (Amersham, Piscataway, NJ). The membranes were then incubated with nonfat dry milk in Tris-buffered saline containing Tween-20 (TBS-T) in order to block non-immunospecific protein binding, and then with a primary antibody against S100 A11 (Santa Cruz, Santa Cruz, CA), and beta-actin (Sigma, St. Louis, MO). After washing with TBS-T, the membranes were incubated with animal-matched HRP-conjugated secondary antibodies (Amersham). Immunoreactivity was visualized with an enhanced chemiluminescence system (Amersham).
Animal matched hrp conjugated secondary antibodies
Manufactured by Cytiva
Animal-matched HRP-conjugated secondary antibodies are laboratory reagents used to detect and visualize target proteins in various immunoassays. These antibodies are conjugated with the enzyme horseradish peroxidase (HRP), which catalyzes a colorimetric or chemiluminescent reaction for signal detection.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Cytiva (2 mentions)
Enhanced chemiluminescence system, by Cytiva (2 mentions)
β actin, by Merck Group (2 mentions)
Smad2 3, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Clic4, by Santa Cruz Biotechnology (1 mentions)
Phosphorylated smad2 3, by Cell Signaling Technology (1 mentions)
2 protocols using animal matched hrp conjugated secondary antibodies
1
Western Blot Analysis of S100 A11
2
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole cell lysates were subjected to SDS-polyacrylamide gel electrophoresis, and transferred onto PVDF membranes (Amersham). The membranes were then incubated with nonfat dry milk in Tris-buffered saline containing Tween-20 (TBS-T) to block non-immunospecific protein binding, and then with a primary antibody against KRAS (Santa Cruz, Santa Cruz, CA), CLIC4 (Santa Cruz), Smad2/3 (Cell Signaling Technology, Danvers, MA), phosphorylated Smad2/3 (Cell Signaling Technology), caspase-3 (Cell Signaling Technology), or β-actin (Sigma). After washing with TBS-T, the membranes were incubated with animal-matched HRP-conjugated secondary antibodies (Amersham). Immunoreactivity was visualized with an enhanced chemiluminescence system (Amersham).
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