Peptone

Disodium cromoglycate (5′DSCG) (98% purity) was obtained from TCI Chemicals (Philadelphia, PA). Tryptone, yeast extract, sodium chloride, Tris base, urea, lauryldimethylamine N-oxide and poly(ethylene) glycol (PEG8000) were purchased from Fisher Scientific (Fair Lawn, NJ) and were used to make Luria Bertani media and buffers. All aqueous solutions were dissolved in deionized water with resistivity greater than 18.2 MΩ cm or deuterium oxide (D₂O, 99.9%, Cambridge Isotope Laboratories, Inc., Andover, MA). Casamino acids (Amresco, Solon, OH), peptone (Bio Basic, Amherst, NY) and casitone (BD Biosciences, MD) were also used. l-amino acids (l-glutamic acid, l-alanine, l-arginine), polyvinylpyrrolidone (PVP, MW ∼ 40 000), polyvinylalcohol (PVA, MW ∼ 9000–10 000), poly-acrylamide (PAAm, MW ∼ 9000–10 000), poly(ethylene) glycol (PEG4000), and proteins (lectin A, esterase, lipase, bovine serum albumin and trypsin) were purchased from Sigma Aldrich (St. Louis, MO). Pseudomonas aeruginosa strain PA1244N3 (pPAC46) was obtained from Dr Castric and Dr Horzempa.^{28,29 (link)}