Triple tof 5600 mass spectrometer system triple tof ms

Liquid chromatography-mass spectrometry system consisted of a Thermo Scientific Vanquish UHPLC system (ThermoFisher Scientific, CA, USA) with Poroshell 120 EC-C18 (2.1 x 100 mm, 2.7 μm) column (Agilent) and a triple ToF 5600+ mass spectrometer system (Triple ToF MS) (SCIEX, Foster City, CA, USA). Triple TOF MS, equipped with a DuosprayTM ion source, was used to complete the high resolution experiment.

This study will apply MS-based metabolomic profiling studies for the detection and identification of potential metabolic biomarkers for acne in the participants’ serum. The liquid chromatography-mass spectrometry system will consist of a Thermo Scientific Vanquish ultra-high-performance liquid chromatography (UHPLC) system (Thermo Fisher Scientific, Sunnyvale, CA) with a triple ToF 5600+ mass spectrometer system (Triple ToF MS) (SCIEX, Foster City, CA) for untargeted metabolite analysis. The UHPLC system with a QTRAP 3200TM (hybrid triple quadrupole/linear ion trap) mass spectrometry system (SCIEX, Foster City, CA). The analytical methods will be developed and optimized for targeted and untargeted analyses of metabolites, such as steroidal hormones (dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, and estradiol), fatty acids (oleic acid; cis, elaidic acid; trans, palmitic acid; saturated), and endogenous small metabolites closely related to energy metabolism and lipid/steroid biosynthesis. Statistical techniques will be used to investigate the particular metabolic changes and entire network of the metabolic pathway profile.