Anti β1 ar

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-β1-AR is a laboratory product for the detection and analysis of beta-1 adrenergic receptors. It functions as a specific antibody that binds to the beta-1 adrenergic receptor, which is a G protein-coupled receptor involved in various physiological processes.

Automatically generated - may contain errors

Lab products found in correlation

Anti caveolin 3, by BD (1 mentions) Anti c fos, by Santa Cruz Biotechnology (1 mentions) Fluorescence conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Image pro plus, by Zeiss (1 mentions) Anti quenching mounting medium, by Thermo Fisher Scientific (1 mentions) Lsm 510 laser confocal fluorescence microscope, by Zeiss (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti at1r, by Santa Cruz Biotechnology (1 mentions) Anti at2r, by Santa Cruz Biotechnology (1 mentions) Anti β2 ar, by Santa Cruz Biotechnology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions)

3 protocols using anti β1 ar

Membrane Fraction Isolation and Western Blotting of β-Adrenergic Receptors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hearts were frozen in situ as above and a membrane fraction was isolated as previously described (Zhou et al., 1998 (link)), with modifications. Hearts were homogenized in hypotonic lysis buffer and the
homogenate was centrifuged at 2,000 G for 10 min. The supernatant was collected and centrifuged at 9,000 G for 20 min, and the
resultant supernatant was centrifuged at 180,000 G for 90 min. Essentially all β₁AR and
β₂AR were recovered in this final pellet. The pellet was reconstituted with RIPA buffer containing
protease inhibitor (Roche) then running buffer for Western blotting (anti-β₁AR (Santa Cruz),
anti-β₂AR (as above) and anti-Caveolin3 (BD Biosciences)).

Hayashi H., Hess D.T., Zhang R., Sugi K., Gao H., Tan B.L., Bowles D.E., Milano C.A., Jain M.K., Koch W.J, & Stamler J.S. (2018). S-nitrosylation of β-arrestins biases receptor signaling and confers ligand independence. Molecular cell, 70(3), 473-487.e6.

+ Open protocol

+ Expand

Brain Perfusion and Immunofluorescence Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mice were perfused intracardiacally with saline first, then with 4% paraformaldehyde in 0.1 M Na₂HPO₄/NaH₂PO₄ buffer (pH 7.5) and the brains were removed. After post-fixation in 4% paraformaldehyde for 4 hours, the samples were stored in 30% sucrose/PBS for 3 days. Brain slices 30 μm thick were incubated in primary antibody (anti-c-Fos 1: 1000, Santa Cruz; anti-β1-AR 1:100, Santa Cruz) at 4°C overnight. After being washed with PBS 3 times, the slices were incubated with fluorescence conjugated secondary antibody at room temperature (1:50000, Jackson ImmunoResearch) for 1 hour. Then the brain sections were rinsed in PBS and mounted with antiquenching mounting medium (Thermo Fisher Scientific). The sections were visualized under a LSM 510 laser confocal fluorescence microscope (Carl Zeiss) and analyzed by Image-Pro Plus. Labeled cells above the same threshold determined from control animals were counted (Trifilieff et al., 2006 ).

Zhu H., Zhou Y., Liu Z., Chen X., Li Y., Liu X, & Ma L. (2017). β1-Adrenoceptor in the Central Amygdala Is Required for Unconditioned Stimulus-Induced Drug Memory Reconsolidation. International Journal of Neuropsychopharmacology, 21(3), 267-280.

+ Open protocol

+ Expand

Western Blot Analysis of Adrenergic Receptors in Rat Renal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted after homogenizing the rat renal cortex, and the concentration was determined with a bicinchoninic acid protein assay kit. The proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to nitrocellulose membranes, which were incubated with the primary antibodies rabbit anti-β₁-AR (1:200), anti-β₂-AR (1:200), anti-β₃-AR (1:200), anti-α_1A-AR (1:200), anti-α_1B-AR (1:200), anti-α_1D-AR (1:200), anti-AT₁R (1:200), anti-AT₂R (1:200), and anti-GAPDH (1:200) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight, followed by incubation with goat anti-rabbit fluorescent (IRDye-conjugated) secondary antibodies (1:10,000; Rockland Immunochemicals, Gilbertsville, PA, USA) for 2 h at room temperature. The images were quantified by the Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA). Levels of proteins were normalized to that of GAPDH.

Peng Y., Li Y., Chen M., Song J., Jiang Z, & Shi S. (2020). High-dose nitrate therapy recovers the expression of subtypes α1 and β-adrenoceptors and Ang II receptors of the renal cortex in rats with myocardial infarction-induced heart failures. BMC Cardiovascular Disorders, 20, 99.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!