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Anti human cd44 pe

Manufactured by BioLegend

Anti-human CD44-PE is a flow cytometry labeling reagent used to detect the expression of the CD44 cell surface marker on human cells. CD44 is a receptor for hyaluronic acid and is involved in cell-cell interactions, cell adhesion, and migration. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD44-positive cells using flow cytometry.

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2 protocols using anti human cd44 pe

1

PDX MDSC Co-culture Induces Cancer Stem Cells

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PDX MC1 cell suspensions were prepared as described above, cleared of mouse stromal cells using mouse cell removal kit (Miltenyi Biotec) and mixed with purified MDSCs (Gr1+) from blood of MC1 tumor bearing mouse and cultured under mammosphere conditions. The human cancer stem cell phenotype CD24CD44+ was determined by flowcytometry with anti-human CD24-PECy7 (eBioscience) and anti-human CD44-PE (Biolegend) and included a mouse MDSC marker staining to facilitate the exclusion of this population by gating during data analysis.
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2

PDX MDSC Co-culture Induces Cancer Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PDX MC1 cell suspensions were prepared as described above, cleared of mouse stromal cells using mouse cell removal kit (Miltenyi Biotec) and mixed with purified MDSCs (Gr1+) from blood of MC1 tumor bearing mouse and cultured under mammosphere conditions. The human cancer stem cell phenotype CD24CD44+ was determined by flowcytometry with anti-human CD24-PECy7 (eBioscience) and anti-human CD44-PE (Biolegend) and included a mouse MDSC marker staining to facilitate the exclusion of this population by gating during data analysis.
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