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Xyla scmos camera

Manufactured by Oxford Instruments
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The Xyla sCMOS camera is a scientific-grade camera designed for demanding imaging applications. It features a large-format sCMOS sensor with high resolution, low noise, and fast readout speeds, providing excellent image quality and performance.

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Lab products found in correlation

630nm red led, by Thorlabs (3 mentions) Apo tirf objective, by Oxford Instruments (2 mentions) Nis a n sim analysis module, by Nikon (2 mentions) High precision coverslips, by Zeiss (2 mentions) Ti microscope, by Nikon (2 mentions) B scope, by Thorlabs (2 mentions) Uplanfl, by Olympus (2 mentions)

Close spelling variants of this product

SCMOS camera

7 protocols using xyla scmos camera

1

Intrinsic Hemodynamic Imaging of Cortical Responses

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Intrinsic signal imaging was performed using a custom intrinsic signal imaging setup with a Xyla sCMOS camera (Andor) controlled by μManager242 . To obtain a blood vessel map, we acquired an image of the cortical surface under white light illumination. To measure intrinsic hemodynamic responses, we illuminated the surface of the cortex with a 630nm red LED (Thorlabs). Visually-driven responses were evoked using a continuously drifting and rotating square-wave grating (0.06 cycles per °, 4 cycles per second, rotated either clockwise or counter-clockwise at 6° per second) and recorded at 50–55 Hz.
Wilson D.E., Whitney D.E., Scholl B, & Fitzpatrick D. (2016). Orientation selectivity and the functional clustering of synaptic inputs in primary visual cortex. Nature neuroscience, 19(8), 1003-1009.
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2

Chromaffin Cell Imaging Protocol

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Isolated chromaffin cells were plated onto high precision coverslips (Zeiss), fixed 3–5 days later and immunostained as described above. Samples were imaged on a Nikon Ti microscope equipped with a 100× 1.49 NA Apo TIRF objective and an Andor Xyla sCMOS camera. Structured illumination images were acquired as a 15-slice z-stack with 120 nm step size and reconstructed using Nikon Elements software outfitted with the NIS-A N-SIM analysis module. Colocalization was quantified with the Coloc2 module in ImageJ.
Logan T., Bendor J., Toupin C., Thorn K, & Edwards R.H. (2017). α-Synuclein Promotes Dilation of the Exocytotic Fusion Pore. Nature neuroscience, 20(5), 681-689.
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3

Cortical Epi-fluorescence Imaging Pipeline

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Epi-fluorescence imaging was performed using a custom light path on the B-Scope with 525 nm LED illumination (Thorlabs). GCaMP6s fluorescence signal from the cortical surface was acquired at about 15 Hz (640x540 pixels, field of view (FOV) ranges from 3x2.53 to 4x3.38 mm2) using a Xyla sCMOS camera (Andor) controlled by μManager2. Average excitation power at the exit of the objective (4x, UPlanFl, Olympus) ranged from 0.2 to 0.8 mW. Epi-fluorescence frame triggers from μManager2 and stimulus events were recorded using Spike2 (CED; Cambridge, UK). The visual stimulus and the analysis method were the same as two-photon pixel based experimental design. Z-projections of two-photon fields of view were aligned to the epi-fluorescence imaging with the blood vessel pattern.
Lee K.S., Huang X, & Fitzpatrick D. (2016). Topology of ON and OFF inputs in visual cortex enables an invariant columnar architecture. Nature, 533(7601), 90-94.
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4

Chromaffin Cell Imaging Protocol

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Isolated chromaffin cells were plated onto high precision coverslips (Zeiss), fixed 3–5 days later and immunostained as described above. Samples were imaged on a Nikon Ti microscope equipped with a 100× 1.49 NA Apo TIRF objective and an Andor Xyla sCMOS camera. Structured illumination images were acquired as a 15-slice z-stack with 120 nm step size and reconstructed using Nikon Elements software outfitted with the NIS-A N-SIM analysis module. Colocalization was quantified with the Coloc2 module in ImageJ.
Logan T., Bendor J., Toupin C., Thorn K, & Edwards R.H. (2017). α-Synuclein Promotes Dilation of the Exocytotic Fusion Pore. Nature neuroscience, 20(5), 681-689.
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5

Intrinsic Hemodynamic Imaging of Cortical Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intrinsic signal imaging was performed using a custom intrinsic signal imaging setup with a Xyla sCMOS camera (Andor) controlled by μManager242 . To obtain a blood vessel map, we acquired an image of the cortical surface under white light illumination. To measure intrinsic hemodynamic responses, we illuminated the surface of the cortex with a 630nm red LED (Thorlabs). Visually-driven responses were evoked using a continuously drifting and rotating square-wave grating (0.06 cycles per °, 4 cycles per second, rotated either clockwise or counter-clockwise at 6° per second) and recorded at 50–55 Hz.
Wilson D.E., Whitney D.E., Scholl B, & Fitzpatrick D. (2016). Orientation selectivity and the functional clustering of synaptic inputs in primary visual cortex. Nature neuroscience, 19(8), 1003-1009.
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6

Cortical Epi-fluorescence Imaging Pipeline

Check if the same lab product or an alternative is used in the 5 most similar protocols
Epi-fluorescence imaging was performed using a custom light path on the B-Scope with 525 nm LED illumination (Thorlabs). GCaMP6s fluorescence signal from the cortical surface was acquired at about 15 Hz (640x540 pixels, field of view (FOV) ranges from 3x2.53 to 4x3.38 mm2) using a Xyla sCMOS camera (Andor) controlled by μManager2. Average excitation power at the exit of the objective (4x, UPlanFl, Olympus) ranged from 0.2 to 0.8 mW. Epi-fluorescence frame triggers from μManager2 and stimulus events were recorded using Spike2 (CED; Cambridge, UK). The visual stimulus and the analysis method were the same as two-photon pixel based experimental design. Z-projections of two-photon fields of view were aligned to the epi-fluorescence imaging with the blood vessel pattern.
Lee K.S., Huang X, & Fitzpatrick D. (2016). Topology of ON and OFF inputs in visual cortex enables an invariant columnar architecture. Nature, 533(7601), 90-94.
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7

Intrinsic Signal Imaging of Visual Cortex

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Intrinsic signal imaging was performed using a custom setup including a Xyla sCMOS camera (Andor) controlled by μManager2 software. Intrinsic hemodynamic responses were obtained by illuminating the surface of the brain with a 630nm red LED (Thorlabs). Maps of azimuth and elevation were generated by stimulating visually evoked responses with a vertical or horizontal black bar placed at a range of offsets from the center of gaze (azimuth: 0 to 30 degrees in 3 degree increments; elevation: −20 to +20 degrees in 4 degree increments). The position of the bar was jittered within +/− 0.5 degrees during the 4 second stimulation.
Schumacher J.W., McCann M.K., Maximov K.J, & Fitzpatrick D. (2022). Selective enhancement of neural coding in V1 underlies fine discrimination learning in tree shrew. Current biology : CB, 32(15), 3245-3260.e5.
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