Approximately 1 × 106 spleen cells were collected, resuspended in 100 μl of PBS and incubated with 1.5 μl of PE-conjugated anti-mouse CD4, PE/Cy5-conjugated anti-mouse CD8a and FITC-conjugated anti-mouse CD3, PE-conjugated anti-mouse CD19 and FITC-conjugated anti-mouse CD80, or FITC-conjugated anti-mouse CD86 antibodies (all from BioLegend) in the dark on ice for 30 min. The cells were then washed and analyzed using a FACSCalibur flow cytometer and CellQuest software (BD Biosciences).
Fitc conjugated anti mouse cd3
Manufactured by BioLegend
FITC-conjugated anti-mouse CD3 is a monoclonal antibody that binds to the CD3 complex on the surface of mouse T cells. The antibody is conjugated with the fluorescent dye FITC, allowing for the detection and identification of T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Pe cy5 conjugated anti mouse cd8a, by BioLegend (1 mentions)
Pe conjugated anti mouse cd19, by BioLegend (1 mentions)
Pe conjugated anti mouse cd4, by BioLegend (1 mentions)
Fitc conjugated anti mouse cd80, by BioLegend (1 mentions)
Fitc conjugated anti mouse cd86, by BioLegend (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
Facsaria 3, by BD (1 mentions)
2 protocols using fitc conjugated anti mouse cd3
1
Multiparametric Flow Cytometry of Mouse Spleen Cells
2
Sorting B Lymphocytes from Splenocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Splenocytes were first obtained as described earlier and then stained with FITC-conjugated anti-mouse CD3 (Biolegend) and PE/Cy5.5-conjugated anti-mouse CD19 (Biolegend). The CD3−CD19+ cells (B lymphocytes) were sorted for further analysis on FACSAriaIII (BD Bioscience) by the Flow Cytometric Analyzing and Sorting Core Facility at National Taiwan University Hospital.
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