Cleaved caspase 3 no 9661

Manufactured by Cell Signaling Technology

Sourced in United States

Cleaved caspase-3 (no. 9661) is an antibody used to detect the cleaved form of caspase-3, a key enzyme involved in the execution phase of cellular apoptosis (programmed cell death). The antibody specifically recognizes the p17 fragment of cleaved caspase-3, providing a reliable marker for the activation of this important apoptotic signaling pathway.

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Lab products found in correlation

Polyvinylidene difluoride membrane, by Thermo Fisher Scientific (1 mentions) Mammalian protein extraction reagent buffer, by Thermo Fisher Scientific (1 mentions) Wet electrophoretic transfer unit, by Bio-Rad (1 mentions) Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Mini protean tgx precast gel, by Bio-Rad (1 mentions) Ab194699, by Abcam (1 mentions) Ab56164, by Abcam (1 mentions) Ab187091, by Abcam (1 mentions) Ab9021, by Abcam (1 mentions) Alexa fluor 488 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) E cadherin ab1416, by Abcam (1 mentions)

Close spelling variants of this product

Cleaved caspase-3 Caspase-3 Caspases

2 protocols using cleaved caspase 3 no 9661

Fludarabine-Induced Protein Signaling in TSC2-null Cells

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Protein lysis of TSC2-null 621-101 cells treated with Fludarabine was done using mammalian protein extraction reagent buffer (Thermo Fisher Scientific, no. 78501) containing protease and phosphatase inhibitors. Lysates were resolved on 4 to 20% Mini-PROTEAN TGX Precast gels (Bio-Rad, no. 4561094) and transferred using a wet electrophoretic transfer unit (Bio-Rad) onto polyvinylidene difluoride membranes (Thermo Fisher Scientific). After 1 hour of blocking with 5% nonfat dry milk, membranes were incubated with primary antibodies at 4°C overnight. Primary antibodies include: phospho-STAT1 (no. 8826), STAT1 (no. 14994), phospho-STAT3 (no. 9145), STAT3 (no. 30835), PBX1 (no. 4342), cleaved caspase-3 (no. 9661), PARP (no. 9532), cyclin D1 (no. 55506), phospho-RIPK1 (no. 44590), and β-actin (no. 3700) from Cell Signaling Technology. Secondary antibody incubation was done using horseradish peroxidase–linked antibodies for 2 hours. Signal was detected with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, no. PI34580).

Olatoke T., Wagner A., Astrinidis A., Zhang E.Y., Guo M., Zhang A.G., Mattam U., Kopras E.J., Gupta N., Smith E.P., Karbowniczek M., Markiewski M.M., Wikenheiser-Brokamp K.A., Whitsett J.A., McCormack F.X., Xu Y, & Yu J.J. (2023). Single-cell multiomic analysis identifies a HOX-PBX gene network regulating the survival of lymphangioleiomyomatosis cells. Science Advances, 9(19), eadf8549.

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Antibody Validation for Cell Death Pathways

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The following antibodies were purchased from Santa Cruz Biotechnology, Dallas, TX, USA: cleaved caspase-8 (sc-1226), RIP3 (sc-47368), ACTB (sc-1616) and α-tubulin (TUBA; sc-31779). Cleaved caspase-3 (no. 9661) and RIP1 (no. 3493) were purchased from Cell Signaling Technology, Danvers, MA, USA. Antibodies specific to PLAP (ab118856), RIP3 (ab56164), total MLKL (ab194699), phospho-MLKL (ab187091 and its competing peptide ab206929), cytokeratin-7 (ab9021), and E-cadherin (ab1416) were purchased from Abcam, Cambridge, UK. GCM1 antibody (P100836_P050) was purchased from Aviva Systems Biology, Corp., San Diego, CA, USA. Secondary antibodies include horseradish peroxidase-conjugated donkey anti-goat, goat anti-rabbit and goat anti-mouse from Santa Cruz Biotechnology. For immunofluorescence, the following secondary antibodies were used: Alexa Fluor 594 donkey anti-rabbit and Alexa Fluor 488 donkey anti-rabbit from Life Technologies, Carlsbad, CA, USA.

Bailey L.J., Alahari S., Tagliaferro A., Post M, & Caniggia I. (2017). Augmented trophoblast cell death in preeclampsia can proceed via ceramide-mediated necroptosis. Cell Death & Disease, 8(2), e2590-.

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