Honokiol (98% purity) was purchased from Nacalai Tesque (Kyoto, Japan). Thiazolyl blue tetrazolium bromide (MTT) and other chemicals of analytical grade were purchased from Sigma Chemical Co. (St. Louis, MO). Leupeptin, pepstatin, and cell proliferation ELISA kit were purchased from Roche Diagnostics GmbH (Mannheim, Germany). Primary antibodies for procaspase 3, caspase 8, caspase 9, CDK6, p53, and cleaved PARP were purchased from Cell Signaling Technology (Beverly, MA). Primary antibodies for cyclin D1, cyclin D2, cyclin E, CDK-2, CDK-4, cyclin A, Cdc2p34, PCNA, caspase 3, anti-mouse IgG horseradish peroxidase-linked and anti-rabbit IgG horseradish peroxidase-linked secondary antibodies, and nitrocellulose membranes were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-Kip1/p27 antibody was purchased from BD-Pharmingen (San Diego, CA), cyclin B1 and anti-Cip1/p21 antibody from Upstate Biotechnology (Lake Placid, NY).
Honokiol
Manufactured by Nacalai Tesque
Sourced in Japan
Honokiol is a natural bioactive compound extracted from the bark and leaves of Magnolia tree species. It is a polyphenolic compound with antioxidant properties. Honokiol can be used as a reference standard in analytical testing and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions)
Nitrocellulose membrane, by Santa Cruz Biotechnology (1 mentions)
Cell proliferation elisa kit, by Roche (1 mentions)
Cdc2 p34, by Santa Cruz Biotechnology (1 mentions)
Leupeptin, by Roche (1 mentions)
Pepstatin, by Roche (1 mentions)
Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions)
Glutamax 1 supplement, by Thermo Fisher Scientific (1 mentions)
6 ohda, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Valley Biomedical (1 mentions)
Hydrogen peroxide, by Kanto Chemical (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Protein assay, by Bio-Rad (1 mentions)
2 protocols using honokiol
1
Honokiol Inhibits Apoptosis and Cell Cycle
2
Neuroprotective Effects of Honokiol and Magnolol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Honokiol and magnolol were purchased from Nacalai Tesque Inc. (Kyoto, Japan). Rat adrenal medulla PC12 cells were obtained from American Type Culture Collection (Manassas, VA, USA). 6-OHDA and RPMI-1640 medium were purchased from Sigma-Aldrich (St Louis, MO, USA). Antibiotic-Antimycotic (100 ×) liquid, horse serum and GlutaMAX TM -I Supplement were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum was purchased from Valley Biomedical (Winchester, VA, USA). The CellTiter-Glo ® Luminescent Cell Viability Assay kit was obtained from Promega Co. (Madison, WI, USA). Hydrogen peroxide was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Cell culture Petri dishes (collagen type I coated ware) and 24-well cell culture multidisc (collagen type I coated ware) were from AGC Techno Glass Co. Ltd. (Shizuoka, Japan). The Bio-Rad Protein Assay was purchased from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). All other reagents were of the highest grade commercially available.
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