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2 protocols using manganese 3 tetrakis 1 methyl 4 pyridyl porphyrin mntmpyp

1

Oxidative Stress Modulation in Cells

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Sodium arsenite (Na2AsO2), apocynin, 5,5-dimethyl-1-pyrroline-1-oxide (DMPO), and Annexin V/Propidium iodide (PI) were purchased from Sigma (St Louis, MO). Both 5-(and -6)-chloromethyl-2, 7-dichlorodihydrofluorescein diacetate, acetyl ester (DCFDA) and dihydroethidium (DHE) were purchased from Molecular Probes (Eugene, OR). Manganese(III) tetrakis(1-methyl-4-pyridyl)porphyrin (MnTMPyP) was purchased from Cayman Chemical (Ann Arbor, MI). Plasmids DNA encoding human catalase and SOD2 and catalase shRNA were purchased from Origene (Rockville, MD). Antibody against SOD2 was purchased from Millipore (Billerica, MA). Antibodies against catalase, C-PARP, C-Caspase-3, and Bcl-2 were from Cell Signaling Tech (Danvers, MA).
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2

Signaling Pathways and Oxidative Stress

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Potassium dichromate (K2Cr2O7), apocynin (APO), 5,5-dimethyl-1-pyrroline-1-oxide (DMPO), were purchased from Sigma-Aldrich (St Louis, MO). Both Dichlorodihydrofluoresceine acetate (DCFDA) and dihydroethidium (DHE) were purchased from Molecular Probes (Eugene, OR). Manganese(III) tetrakis(1-methyl-4-pyridyl) porphyrin (MnTMPyP) was purchased from Cayman Chemical (Ann Arbor, MI). Human miR-21 hairpin inhibitor was purchased from Thermo Fisher Scientific Dharmacon (Chicago, IL, USA). Antibodies against PDCD4 (CST#9535), E-cadherin (CST#3195), β-catenin (CST#8480), c-Myc (CST#5605), uPAR (CST#9692), pSTAT3 Tyr705 (CST#9145) and STAT3 (CST#9139) were purchased from Cell Signaling Technology (Danvers, MA). Anti–active-β-catenin (clone 8E7#05-665) antibody was purchased from EMD Millipore (Billerica, MA). Antibodies against TCF4 (sc-13027) and GAPDH (sc-25778) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).
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