Accu check aviva meter

At 3 weeks of age, mice were weaned and group-housed with littermates of the same sex. At 4 weeks of age, weight gain was measured regularly on a weekly basis until 15 weeks of age using a Sartorius ELT402 balance. Length was measured on anesthetized mice (short induction with isoflurane gas) at 16–17 weeks old by manual immobilization and extension of the mice to their full length and measurement of the nose-to-anus distance in centimeters. Whole blood was collected by tail vein bleeding in fasted mice (5 hours fasting prior to bleeding) at 16–17 weeks of age, and blood glucose was assessed using Accu-check Aviva meter (Roche). Basal food intake on regular chow diet (Teklad Global 18% Protein Rodent Diet from Harlan Laboratories) was measured in individualized mice of 19–20 weeks of age. Mice were individually housed at least 4 days before any measurements were taken. A sufficient amount of food for the week was then weighed and provided to the mice ad libitum. Each day, morning (8 am) and afternoon (5 pm) at the same time, the remaining food was measured for 5 consecutive days. The daily average of food intake during dark cycle and light cycle was calculated for each genotype. The caloric intake was estimated using 3.1 kcal/g as metabolizable energy.

Animals were fasted for 6 h and blood glucose was measured via taking one drop of blood from the animal tails after cutting the very tip using an Accu-Check® Aviva meter (Roche, Basel, Switzerland) every 15 min following intraperitoneal administration of 2 g/kg glucose or 0.2 g/kg pyruvate for 2 h.