Log In Sign up
The largest database of trusted experimental protocols

Tg101348

Manufactured by Selleck Chemicals
Visit Supplier
Sourced in United States

TG101348 is a chemical compound used in laboratory settings. It is a crystalline solid that is soluble in organic solvents. The core function of TG101348 is to serve as a research tool for scientific investigations, but its specific intended use should not be extrapolated.

Automatically generated - may contain errors

Lab products found in correlation

Azd1480, by Selleck Chemicals (3 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Bms911543, by Chemietek (1 mentions) Mitomycin c, by Selleck Chemicals (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Rock inhibitor y 27632, by Bio-Techne (1 mentions) Idarubicin, by Selleck Chemicals (1 mentions) Purvalanol a, by Selleck Chemicals (1 mentions) Wz3146, by Selleck Chemicals (1 mentions) Azd8055, by Selleck Chemicals (1 mentions) Cck 8 kit, by Dojindo Laboratories (1 mentions) Celltox green cytotoxicity assay, by Promega (1 mentions) Fludarabine, by Selleck Chemicals (1 mentions) Cellavista, by Roche (1 mentions) Amlexanox, by Bio-Techne (1 mentions) Ruxolitinib, by Selleck Chemicals (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Ifn γ, by R&D Systems (1 mentions) Griess reagent system, by Promega (1 mentions) Incb018424, by Selleck Chemicals (1 mentions)

11 protocols using tg101348

1

Sialidase-Modulated Platelet-Induced STAT3 Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
HepG2 cells (purchased from ATCC-HB-8065) were grown at 4×105 cells/cm2 in Permanox® (Nalge Nunc International) slides for 24 h in the presence of 2 μM AZD1480 or 10 μM of TG101348 (both Selleckchem) or 2 μM of BMS911543 (Chemie Tek) or vehicle before addition of human platelets treated or non-treated with sialidase. Immunofluorescence for anti-pSTAT3 (Cell Signaling Technology) was performed 1 h after platelet addition to HepG2 cells following antibody manufactor’s protocol. After 6 h incubation with humans platelets treated or non-treated with sialidase, HepG2 cells were fixed in 4% PFA, followed by permeabilization in 2% Triton X-100. Cells were incubated for 30 min at 37 °C with 5% goat-serum to block unspecific binding and incubated overnight at 4 °C with a polyclonal anti-human TPO antibody (R&D Systems, USA) followed by secondary AlexaFluo®568 conjugated polyclonal goat-anti rabbit antibody (Invitrogen). Nuclei were visualized by inclusion of 4′, 6-diamino-2-phenyllidole (DAPI) (Invitrogen) in the mounting medium.
Grozovsky R., Begonja A.J., Liu K., Visner G., Hartwig J.H., Falet H, & Hoffmeister K.M. (2014). The Ashwell-Morell receptor regulates hepatic thrombopoietin production via JAK2-STAT3 signaling. Nature medicine, 21(1), 47-54.
+ Open protocol
+ Expand
2

Cell Viability Assay for OCI-Ly3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The JAK2-selective inhibitor TG101348 (Wernig et al., 2008 (link)) and Mitomycin C were purchased from Selleckchem and Tocris Bioscience respectively and were dissolved in DMSO. OCI-LY3 cells were treated with the indicated compounds in 96-well plates and their growth was determined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega Corp). Typically, 30,000 OCI-Ly3 cells per well in 200 µL of growth medium were grown for 48h in the presence or absence (DMSO alone) of the desired compounds, and then assayed with CellTiter Glo according to manufacturer’s instructions.
Woo J.H., Shimoni Y., Yang W.S., Subramaniam P., Iyer A., Nicoletti P., Martínez M.R., López G., Mattioli M., Realubit R., Karan C., Stockwell B.R., Bansal M, & Califano A. (2015). Elucidating Compound Mechanism of Action by Network Perturbation Analysis. Cell, 162(2), 441-451.
+ Open protocol
+ Expand
3

Isolation and Culture of Mouse TCRgd Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pooled LN from 5 to 10 mice (12 to 24 weeks) were mechanically disaggregated and incubated with a biotin-coupled anti-TCRγδ antibody (GL3; BD Pharmingen) followed by incubation with Streptavidin Microbeads. TCRγδ cells were isolated by positive selection using AutoMacs or MS columns (Miltenyi Bitotec); 50% to 80% purity of TCRγδ cells was typically obtained with this method. Isolated TCRγδ cells were cultured in IMDM-10%-2ME supplemented with IL-7 (5 ng/mL; Prepotec) for 5 to 10 days. IL-7 was refreshed every 3 days. Cells were treated with the following inhibitors: JAK2 inhibitors AZD1480 (5 μM; SelleckChem) and TG101348 (2 μM; SelleckChem), ROCK inhibitor Y27632 (10–20 μM; Tocris), and MLCK inhibitor ML7 (10 μM; Tocris).
Álvarez-Salamero C., Castillo-González R., Pastor-Fernández G., Mariblanca I.R., Pino J., Cibrian D, & Navarro M.N. (2020). IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics. PLoS Biology, 18(3), e3000646.
+ Open protocol
+ Expand
4

Cytotoxicity Evaluation of Anticancer Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human neuroblastoma cell line SH-SY5Y was cultured in RMPI 1640 medium containing 15% fetal bovine serum (FBS). Cells were seeded into 96-well plates (10 000 per well), treated for 24 h with different concentration of purvalanol A, idarubicin, WZ3146, AZD-8055, TG-101348 (10-8~10-5 mol/L, all purchased from Selleck, Shanghai, China). The cells were incubated at 37 °C for 2 h with CCK-8 Kit (Dojindo, Tokyo, Japan). Medium without cells was incubated with CCK8 as blank well. The optical densities (ODs) were checked at 450 nm using a microplate reader. The inhibition rate was calculated as following: (ODdrug-ODblank)/(ODcontrol-ODblank) × 100%.
Ma P., Yue L., Zhang S., Hao D., Wu Z., Xu L., Du G, & Xiao P. (2020). Target RNA modification for epigenetic drug repositioning in neuroblastoma: computational omics proximity between repurposing drug and disease. Aging (Albany NY), 12(19), 19022-19044.
+ Open protocol
+ Expand
5

Microglia and Monocyte Cell Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
BV-2 female mouse microglia-like cells were grown in DMEM + 10% FBS, and THP-1 male human monocyte cells were grown in RPMI-40+ 10% FBS+ 0.05mM β-mercaptoethanol at 37°C, 5% CO2. Adherent cells were split using 1X TrypLE (Gibco). For cellular assays, cells were treated with the following concentrations of drugs, unless otherwise noted, in DMEM (for BV-2) or RPMI-40 (for THP-1) without serum: 100-200μM ganciclovir, 10ng/ml IFNγ (R&D systems), 100ng/ml LPS (Sigma-Aldrich), 10μM Fludarabine (Selleckchem), 1μM Ruxolitinib (Selleckchem), 1μM TG-101348 (Selleckchem), 1μM Amlexanox (Tocris bioscience). Secreted signaling proteins were measured in conditioned culture supernatants from BV-2 cells stimulated with GCV for 24h in the absence of serum using two independent Luminex arrays (Human Immune Monitoring Center, Stanford University and Eve technologies, Canada). Nitrite assay was performed on conditioned culture supernatants of cells stimulated with drugs for 24h using the Griess Reagent System (Promega) according to manufacturer’s instructions. To assess cell viability, cell confluence was measured using an automated microscope (Cellavista; Roche). Toxicity was measured using Celltox Green cytotoxicity assay (Promega). All experiments were run in triplicates and replicated at least 3 times with cell lines and at least twice with primary microglia.
Mathur V., Burai R., Vest R.T., Bonanno L.N., Lehallier B., Zardeneta M., Mistry K.N., Do D., Marsh S.E., Abud E.M., Blurton-Jones M., Li L., Lashuel H.A, & Wyss-Coray T. (2017). Activation of the STING-dependent type I interferon response reduces microglial reactivity and neuroinflammation. Neuron, 96(6), 1290-1302.e6.
+ Open protocol
+ Expand
6

Compound Preparation for Injection

Check if the same lab product or an alternative is used in the 5 most similar protocols
INCB018424, TG101348, and AZD1480 were purchased from Selleck Chemicals (Houston, TX) and prepared for injection as described in the manufacturer’s instructions.
Choi J., Cooper M.L., Alahmari B., Ritchey J., Collins L., Holt M, & DiPersio J.F. (2014). Pharmacologic Blockade of JAK1/JAK2 Reduces GvHD and Preserves the Graft-Versus-Leukemia Effect. PLoS ONE, 9(10), e109799.
+ Open protocol
+ Expand
7

NSCLC Cell Lines and STAT3 Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
The NSCLC PC-9 (EGFR19del), H1650 cells (bearing a deletion in exon 19 of the EGFR gene, i.e., DE746-A750, EGFR19delE746-A750) and H1975 cells (EGFR L858R/T790M, EGFRExon21L858R+T790M+ Exon20T790M) (ATCC, Rockville, MD, USA) [31 (link)] were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 100U/ml penicillin and 100 μg/ml streptomycin (Gibco BRL, Life Technologies, NY). TG101348 and erlotinib were purchased from Selleck Chemicals (Houston, TX, USA). For generation of STAT3-knockdown stable cells, H1975 cells were infected with Lentivirus-STAT3-ShRNA particles and Lentivirus-Ctrl-ShRNA particles (Santa Cruz Biotech., Santa Cruz, CA, USA). Stable clones were selected by puromycin and confirmed by Western blot.
Zhang F.Q., Yang W.T., Duan S.Z., Xia Y.C., Zhu R.Y, & Chen Y.B. (2015). JAK2 inhibitor TG101348 overcomes erlotinib-resistance in non-small cell lung carcinoma cells with mutated EGF receptor. Oncotarget, 6(16), 14329-14343.
+ Open protocol
+ Expand
8

Rabbit MSC Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbits aged 15 weeks, xylazine, ketamine and enrofloxacin, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), phosphate buffered saline (PBS), and double antibodies (Gibco; Thermo Fisher Scientific, Inc.), cluster of differentiation (CD) 45-PE, CD90-PE, CD105-PE, JAK2, phosphorylated (p)-JAK2, p-STAT3 and β-actin antibodies (Abcam), TG101348 (Selleck Chemicals), and 0.22 µm pinhole filter (EMD Millipore).
Wang P, & Zhang Z. (2020). Bone marrow-derived mesenchymal stem cells promote healing of rabbit tibial fractures via JAK-STAT signaling pathway. Experimental and Therapeutic Medicine, 19(3), 2310-2316.
+ Open protocol
+ Expand
9

Synthesis and Characterization of Key Biomolecules

Check if the same lab product or an alternative is used in the 5 most similar protocols
CXCL12 was synthesized using tBoc solid-phase chemistry (39 (link)). Full-length HMGB1 was produced and stored in buffer containing DTT (40 (link)) at the Institute of Research in Biomedicine protein facility, or purchased (1690-HMB, R&D Systems, Minneapolis, MN). PGE2 (14010) was purchased from Cayman Chemical (Ann Arbor, MI). Recombinant human IL-6 (6206-IL) and recombinant human IL-1β (201-LB) were purchased (R&D Systems, Minneapolis, MN). TG101348 was from Selleck Chemicals (S2736, Houston, TX), celecoxib (PZ0008), and Auranofin (A6733) were purchased from Sigma-Aldrich (Saint Louis, MO). Mouse anti-human CXCR4 APC-conjugated (555976, BD Biosciences, San Jose, CA), mouse anti-human CXCL12 (MAB350, R&D Systems, Minneapolis, MN), rabbit anti-human HMGB1 (Ab18256, Abcam, Cambridge, UK), mouse anti-HMGB1 (HM-901, HMGBiotech, Milan, Italy), and rabbit anti-human thioredoxin (2429, Cell Signaling technology, Leiden, The Netherlands).
Cecchinato V., D'Agostino G., Raeli L., Nerviani A., Schiraldi M., Danelon G., Manzo A., Thelen M., Ciurea A., Bianchi M.E., Rubartelli A., Pitzalis C, & Uguccioni M. (2018). Redox-Mediated Mechanisms Fuel Monocyte Responses to CXCL12/HMGB1 in Active Rheumatoid Arthritis. Frontiers in Immunology, 9, 2118.
+ Open protocol
+ Expand
10

Investigating JAK2/STAT3 Signaling in IL-6 Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
WP1066 and TG101348 (Selleckchem) were used in this study. Anti-recombinant human interleukin-6 (anti-rh IL-6), rh interleukin-6 (rh IL-6) and normal goat IgG were purchased from R&D Systems (R&D Systems, MN). JAK2, pJAK2 (Y1007), STAT3, pSTAT3 (Y705) and N-cadherin were purchased from Cell signaling (Danvers, MA). Snail, E-cadherin, Vimentin, GAPDH and IL-6R were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). α-tubulin antibody was obtained from Sigma-Aldrich. Alexa Fluor conjugated anti-rabbit antibody was obtained from Invitrogen (Carlsbad, CA).
Kim S., Gwak H., Kim H.S., Kim B., Dhanasekaran D.N, & Song Y.S. (2016). Malignant ascites enhances migratory and invasive properties of ovarian cancer cells with membrane bound IL-6R in vitro. Oncotarget, 7(50), 83148-83159.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!