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Riboflavin 5 monophosphate sodium salt riboflavin

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Riboflavin 5'-monophosphate sodium salt (riboflavin) is a biochemical compound used in laboratory research. It is the sodium salt form of riboflavin 5'-monophosphate, which is the coenzyme form of vitamin B2. This compound is commonly used as a component in cell culture media and various biochemical assays.

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5 protocols using riboflavin 5 monophosphate sodium salt riboflavin

1

Photocurable Osteosarcoma Drug Delivery

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GC (≥60% calculated by titration, crystalline, MW ≈ 585,000 g/mol) and glycidyl methacrylate (GM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Riboflavin 5′-monophosphate sodium salt (riboflavin; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) was used for photo-curing. Doxorubicin·hydrochloride (DOX·HCl; Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) and cisplatin (CP; Sigma-Aldrich, St. Louis, MO, USA) were used for OSA therapy. (2-hydroxypropyl)-beta-cyclodextrin (HP-β-CD; St. Louis, MO, USA) was used to improve the water solubility of CP. Cellulose membrane (cut-off: 20 kDa) was purchased from Spectrum Laboratories Inc. (Rancho Dominguez, CA, USA). MG-63 and KHOS/NP human osteosarcoma cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). A cell counting kit-8 (CCK-8; Dojindo Molecular Technologies, Inc. Rockville, MD, USA) was used for in vitro cell viability assay. All chemicals were used as received.
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2

Photocurable GC-GM-β-CD-PTX Hydrogel

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GC (≥60% calculated by titration, crystalline, MW ≅ 585,000 g/mol), glycidyl methacrylate (GM), β-CD, and paclitaxel (PTX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Riboflavin 5′-monophosphate sodium salt (riboflavin; Santa Cruz Biotechnology, Inc.; Santa Cruz, CA, USA) was used for photo-curing. The cellulose membrane was purchased from Spectrum Laboratories Inc. (Rancho Dominguez, CA, USA). Human OC cell line SKOV3 purchased from the American Type Culture Collection (Manassas, VA, USA) was used for the in vitro cell viability rate and in vivo animal tests. All chemicals were used as received without further purification.
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3

Visible-Light-Cured Hydrogel for Cancer Therapy

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GC (≥60% calculated by titration, crystalline, MW ≈ 585,000 g/mol) and glycidyl methacrylate (GM) for visible-light-cured hydrogel preparation were purchased from Sigma-Aldrich (St. Louis, MO, USA). Riboflavin 5′-monophosphate sodium salt (riboflavin) used for photo curing was supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Doxorubicin hydrochloride (DOX⋅HCl; Tokyo Chemical Industry Co., Ltd, Tokyo, Japan) and paclitaxel (PTX; Shin Poong Pharm, Co., Ltd.; Ansan, Kyunggi, Republic of Korea) were used for breast cancer therapy. β-CD (Sigma-Aldrich; St. Louis, MO, USA) was used to improve the water solubility of PTX. The cellulose membrane was purchased from Spectrum Laboratories Inc. (Rancho Dominguez, CA, USA) and theMCF-7 human breast cancer cell line was obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). Cell counting kit-8 (CCK-8; Dojindo Molecular Technologies, Inc. Rockville, MD, USA) was used for the in vitro cell viability assay. All chemicals were used as received.
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4

Photocrosslinkable Methacrylated Glycol Chitosan-Grafted CD

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GC (≥60%, crystalline, Mw ≅ 585,000 g/mol) and glycidyl methacrylate (GM) were purchased from Sigma-Aldrich (St. Louis, MO, USA) for MGC preparation. β-CD-COOH (St. Louis, MO, USA) was used for MGC-g-CD preparation. Riboflavin 5′-monophosphate sodium salt (riboflavin; Santa Cruz, CA, USA) was used as a photoinitiator. 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morphoninium chloride (DMT-MM) as a condensing agent was supplied by Wako Pure Chemical Industries (Osaka, Japan). TCS obtained from Sigma-Aldrich (St. Louis, MO, USA) was used as an antibacterial agent. As a cyanoacrylate adhesive, Liquiband® Surgical S (Advanced Medical Solutions Group Plc, Cheshire, UK) was used. MGC-g-CD was purified using a dialysis membrane tube (cut-off: 25 kDa; Spectrum Laboratories Inc., Rancho Dominguez, CA, USA). Mouse fibroblast L-929 cell line was supplied by the Korean Cell Line Bank (Seoul, Korea). Chemicals were used as received.
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5

Methacrylated Collagen Hydrogel Synthesis

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Collagen was purchased from MSBIO, Inc. (Seongnam, Kyunggi, Korea). Methacrylic anhydride (MA), sodium phosphate monobasic, sodium phosphate dibasic, 2-hydroxypropyl-beta-cyclodextrin (HP-β-CD), and triclosan (TCS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Riboflavin 5′-monophosphate sodium salt (riboflavin) as a photoinitiator was obtained from Santa Cruz (Santa Cruz, CA, USA). Dialysis membrane tubes (cut-off: 25 kDa and 3.5–5.0 kDa; Spectrum Laboratories Inc., Rancho Dominguez, CA, USA) were used for methacrylated collagen (ColMA). The NIH3T3 mouse fibroblast cell line was obtained from the Korean Cell Line Bank (Seoul, Korea).
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