Si snhg16

Two human MM cell lines (RPMI-8226 and NCI-H929) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). MM cell lines were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) and 1% penicillin–streptomycin (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C in a humidified atmosphere of 5% CO₂. MiR-342-3p mimics, miR-342-3p inhibitor, miR-negative control (miR-NC), siRNAs to SNHG16 (si-SNHG16), and si-NC were designed and commercially constructed by Genechem (Shanghai, China). All transfections were conducted using Lipofectamine 2000 (Invitrogen). The sequences used were as follows: si-SNHG16, 5′-GGAACAUACUGCUAUCAUAGA-3ʹ; si-NC, 5′-UUCUCCGAACGUGUCACGUTT-3ʹ; miR-342-3p mimics: 5′-UCUCACACAGAAAUCGCACCCGU-3ʹ; miR-NC: 5′-UUCUCCGAACGUGUCACGUTT-3ʹ; miR-342-3p inhibitor: 5′-ACGGGUGCGAUUUCUGUGUGAGA-3ʹ; NC inhibitor: 5′-CAGUACUUUUGUGUAGUACAA-3ʹ.

Human MM cells RPMI-8226 (Cell Bank of the Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere of 5% CO₂. Overexpression of SNHG16 (OE-SNHG16) and negative control (OE-NC) vectors, siRNAs to SNHG16 (si-SNHG16), and si-NC were purchased from GeneChem (Shanghai, China). All transfections were carried out using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA) in accordance with the manufacturer's instructions.