Column chromatography was carried out using Merck Kieselgel PF254 silica gel, Art. No. 1.07734.1000. Thin-layer chromatography (TLC) was done using Merck DC-Plasticfolie TLC plastic sheet pre-coated with Kieselgel 60 PF (Darmstadt, Germany). Dimethylsulfoxide, hexane, chloroform, acetone, methanol, water, acetonitrile, formic acid, acarbose were purchased from Sigma Aldrich (Saint Louis, MO, USA). All solvents used for LC-MS/MS analysis were HPLC grade; others were analytical grade. The DPP-4 inhibitory screening kit was purchased from Cayman Chemical (Ann Arbor, MI, USA). α-Amylase was purchased from Megazyme (County Wicklow, Ireland).
Dpp 4 inhibitory screening kit
Manufactured by Cayman Chemical
Sourced in United States
The DPP-4 inhibitory screening kit is a laboratory tool designed to assess the inhibitory activity of compounds against the enzyme dipeptidyl peptidase-4 (DPP-4). This kit provides a standardized method for researchers to evaluate the potential of various substances to inhibit the DPP-4 enzyme, which is relevant in various areas of study.
Automatically generated - may contain errors
Lab products found in correlation
Kieselgel pf254, by Merck Group (2 mentions)
Dc plasticfolie tlc plastic sheet, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Acarbose, by Merck Group (2 mentions)
α amylase, by Megazyme (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Dimethyl sulphoxide dmso, by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
2 protocols using dpp 4 inhibitory screening kit
1
Chromatographic Separation and Enzymatic Analysis
2
Chromatographic Separation and Bioactivity Analysis
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Column chromatography (CC) was carried out using silica gel Merck Kieselgel PF254 Art. No. 1.07734.1000 and silica gel Merck Kieselgel PF254 Art. No. 9385.1000. Thin layer chromatography (TLC) was performed using Merck DC-Plasticfolie TLC plastic sheet pre-coated with Kiecelgel 60 PF (Merck, Kenilworth, NJ). Acarbose, dimethylsulphoxide (DMSO), ascorbic acid, α-tocopherol, butylated hydroxytoluene (BHT), hexane, ethyl acetate (EtOAc), chloroform (CHCl3), methanol (MeOH), and deuterated solvents (chloroform and acetone) were purchased from Sigma Aldrich (St. Louis, MO). α-Amylase was purchased from Megazyme (Co. Wicklow, Ireland). DPP-4 inhibitory screening kit was purchased from Cayman Chemical (Ann Arbor, MI).
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