Histamine
Histamine is a laboratory equipment product used for the detection and quantification of histamine, a naturally occurring chemical compound. It serves as a tool for researchers and scientists to analyze and measure histamine levels in various samples. The core function of this product is to provide accurate and reliable histamine detection and measurement capabilities for scientific investigations.
Lab products found in correlation
13 protocols using histamine
Cellular Signaling Pathway Assay
Synthesis of Molecularly Imprinted Polymers
In Vitro Electrochemical Sensing Setup
Electrochemical Sensor for Histamine Detection
Purified diamine oxidase (DAO) from porcine kidney was obtained from Sigma Aldrich (St. Louis, MO, USA). DAO (7 wt % as dietary supplement) was obtained from Swanson Health Products, Inc. (Fargo, ND, USA). The formulation contained microcrystalline cellulose, sucrose, ascorbic acid, rice starch, hydrated magnesium silicate, corn starch, carboxymethylcellulose, titanium dioxide, and glycerol (proprietary concentrations). Kapton (polyimide) tape, of approximately 30.4 μm film thickness and 50 mm width, was obtained from McMaster-Carr Co. (Elmhurst, IL, USA), silver/silver chloride ink for screen printing was acquired from DuPont (Wilmington, DE, USA). Nine volt batteries were acquired from a local hardware store.
HUVEC Isolation and Activation Protocol
HUVEC were activated with 1 ng/mL IL-1β (Fitzgerald) in cell media 4 h before use in flow experiments. HUVEC were also activated with 100 µM histamine (Acros Organics) 2 min before use in flow experiments.
For E-selectin blocking experiments, activated HUVEC were blocked with 20 µg/mL anti-E-selectin (R&D Systems #BBA26) or IgG1 isotype control (BioLegend #400102) for 30 min before use in flow experiments to prevent leukocyte adhesion.
Histidine Metabolism Pathway Analysis
F I G U R E 1 Metabolic pathways involving measured metabolites from the histidine metabolism L-histidine was from VWR International Ltd (UK). Other chemicals used for measuring enzymatic activities were BugBuster Protein Extraction Reagent (Novagen, Darmstadt, Germany), magnesium chloride, reduced glutathione, pyridoxal-5 0 -phosphate and aminoguanidine (Sigma-Aldrich, Chemie GmbH).
Intracellular Calcium Assay for AsPC-1 Cells
calcium concentration
of AsPC-1 cells was measured using the FLIPR-4 calcium assay reagent
(Cat # R8142, Molecular Devices, San Jose, CA). In brief, cells were
plated in black-walled clear-bottom 96-well plates overnight at ∼80%
confluency using media and conditions described in
for 1 h at 37 °C, 5% CO2 in FLIPR-4 loading buffer,
consisting of FLIPR-4 reagent diluted (as per the manufacturer’s
instructions) in Hank’s balanced salt solution (HBSS) (with
calcium and magnesium) buffered with 20 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid and 0.2% bovine serum albumin,
with pH adjusted to 7.4. Loading buffer also contained probenecid
(2.50 mM, Sigma-Aldrich, Cat # P8761) to prevent leakage of the calcium
reagent from the cells. Calcium response was then measured via a FlexStation
3 Multi-Mode Microplate Reader (Molecular Devices). GPCR agonists
were added, and response in relative fluorescence units was measured
over 105 s for each well, yielding data for peak response and kinetics
of response. For calcium assays and wound-healing assays, we used
the following GPCR agonists: Neurotensin (Cat # 1909, Tocris, Minneapolis,
MN); 2-Thio-UTP (Cat # 3280, Tocris); histamine (Cat # AAJ6172703,
Fischer Scientific); oxytocin (Cat # 1910, Tocris); and sulprostone
(Cat # 14765, Cayman Chemical).
Intracellular Calcium Signaling Assay
Measuring Gastric pH Under Histamine Stimulation
Comprehensive Reagent Acquisition Protocol
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