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Gata1 pe

Manufactured by Cell Signaling Technology

GATA1-PE is a fluorescently-labeled antibody that targets the GATA1 transcription factor. It is designed for use in flow cytometry applications to detect and analyze GATA1 expression in cells.

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2 protocols using gata1 pe

1

Cell Surface Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell surface flow cytometry, cells were incubated with human Fc receptor binding inhibitor (No. 14-9161-73, eBioscience, San Diego, CA) followed by primary antibodies CD235-APC (No. 306607, BioLegend, San Diego, CA), CD41-FITC (RRID: AB_314373, BioLegend No. 303703), and CD11b-PE/Cy5 (No. 101209, RRID: AB_312995, BioLegend) or CD-71-APC (No. BD551374, RRID: AB_398500, BD Biosciences, Franklin Lakes, NJ), CD34-Fluor450 (No. 48-0341-82, RRID: AB_468936, eBioscience), CD34-PE/Cy7 (No. 343615, RRID: AB_2629725, BioLegend), and GATA1-PE (No. 13353, RRID: AB_2798187, Cell Signaling Technology, Danvers, MA). Data were collected on a DxP10 flow cytometer (Cytek, Cerritos, CA) and analyzed using FlowJo Software, Version 9.7.2.
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2

Cell Surface Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell surface flow cytometry, cells were incubated with human Fc receptor binding inhibitor (No. 14-9161-73, eBioscience, San Diego, CA) followed by primary antibodies CD235-APC (No. 306607, BioLegend, San Diego, CA), CD41-FITC (RRID: AB_314373, BioLegend No. 303703), and CD11b-PE/Cy5 (No. 101209, RRID: AB_312995, BioLegend) or CD-71-APC (No. BD551374, RRID: AB_398500, BD Biosciences, Franklin Lakes, NJ), CD34-Fluor450 (No. 48-0341-82, RRID: AB_468936, eBioscience), CD34-PE/Cy7 (No. 343615, RRID: AB_2629725, BioLegend), and GATA1-PE (No. 13353, RRID: AB_2798187, Cell Signaling Technology, Danvers, MA). Data were collected on a DxP10 flow cytometer (Cytek, Cerritos, CA) and analyzed using FlowJo Software, Version 9.7.2.
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