Prominence lc 20a uflc stack hplc system

The different ginseng extracts (TGE, HPG, LPG, and NGE) and twenty-one ginsenoside reference standards were prepared about 1 mg/mL using deionized water or 70% methanol, and then filtered through a 0.22-mm syringe filter before HPLC injection. The HPLC analysis, including validated calibration curves, was conducted as a previous study [42 (link)]. The HPLC instrument used in this study was a Shimadzu Prominence LC-20A UFLC Stack HPLC system (Shimadzu, Kyoto, Japan) equipped with a DGU-20A3 degasser, LC-20AD pump, CTO column oven, SPD-20A detector, and SIL-20A autosampler. A Waters Symmetry Shield RP18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of deionized water (A) and acetonitrile (B). The gradient elution program was as follows: 0–15 min, 20% B; 15–25 min, 20–31% B; 25–35 min, 31–35% B; 35–40 min, 35–36% B; 40–45 min, 36–39% B; 45–75 min, 39–95%; 75–85 min, 95–20% B, 85–90 min, 20% B. The column temperature was set at 25 °C and the flow rate was 1 mL/min. The sample injection volume was 20 μL and the wavelength of detection was set at 203 nm.

The HPLC instrument used in this study was a Shimadzu prominence LC-20A UFLC stack HPLC system (Shimadzu, Kyoto, Japan) equipped with a DGU-20A3 degasser, LC-20AD pump, CTO column oven, SPD-20A detector, and SIL-20A autosampler. A Waters Symmetry Shield RP18 column (4.6 mm × 250 mm, 5 μm, 100 Å) was used. The mobile phase consisted of deionized water (A) and acetonitrile (B). The gradient elution program was as follows: 0–15 min., 20% B; 15–25 min., 20–31% B; 25–35 min., 31–35% B; 35–40 min., 35–36% B; 40–45 min., 36–39% B; 45–75 min., 39–95%; 75–85 min., 95–20% B, and 85–90 min., 20% B. The column temperature was set at 25 °C and the flow rate was 1 mL/min. The sample injection volume was 20 μL and the wavelength of detection was set at 203 nm.