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Tacha s automated hematoxylin

Manufactured by Biocare Medical
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Tacha's Automated Hematoxylin is a laboratory equipment designed for the automated staining of histological samples. It utilizes hematoxylin, a commonly used dye in histology, to stain cellular structures for microscopic examination.

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Lab products found in correlation

Nuclear decloaker, by Biocare Medical (2 mentions) Lot 161509, by Thermo Fisher Scientific (2 mentions) Mach 3 rabbit hrp polymer detection kit, by Biocare Medical (2 mentions) Ab2524, by Abcam (1 mentions) Ab3355, by Abcam (1 mentions) Peroxidazed 1, by Biocare Medical (1 mentions) Mach 1 universal hrp polymer kit, by Biocare Medical (1 mentions) Diva decloaker 20x, by Biocare Medical (1 mentions)

3 protocols using tacha s automated hematoxylin

1

Immunohistochemical Analysis of p53 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed on 4 μm sections that were
cut from FFPE (formalin-fixed, paraffin-embedded) blocks. Sections were then
deparaffinised with xylene and rehydrated through graded ethanol. Antigen
retrieval was achieved with a pH 9.5 Nuclear Decloaker (Biocare Medical) in a
Decloaking pressure cooker at 95°C for 40 min. Tissue sections were then
treated with 3% hydrogen peroxide (LOT 161509; Fisher Chemical) and with
Background Sniper (Biocare Medical, Concord, CA, USA) to reduce nonspecific
background staining. Primary antibody for p53 (Cell Signaling, 2527) was applied
in a 1:150 dilution for 80 min followed by detection with the MACH 3 Rabbit HRP-
Polymer Detection kit (Biocare Medical). Visualization was achieved using VECTOR
NovaRED (SK-4800; Vector Laboratories, Inc.) as chromogen. Lastly, sections were
counterstained with Tacha's Automated Hematoxylin (Biocare Medical).
Mai W.X., Gosa L., Daniels V.W., Ta L., Tsang J.E., Higgins B., Gilmore W.B., Bayley N.A., Harati M.D., Lee J.T., Yong W.H., Kornblum H.I., Bensinger S.J., Mischel P.S., Rao P.N., Clark P.M., Cloughesy T.F., Letai A, & Nathanson D.A. (2017). Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nature medicine, 23(11), 1342-1351.
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2

Immunohistochemical Analysis of Blood Group Antigens

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Samples from human parathyroid glands (n = 3), and thyroid glands (n = 3) were fixed in buffered 4% paraformaldehyde, dehydrated, and embedded in paraffin. Subsequently, 4 µm sections were mounted on Superfrost Plus glass slides (Menzel) and microwave treated for antigen retrieval. Immunostaining was performed after pretreatment with Diva Decloaker 20X (Biocare Medical, Pacheco, CA, USA) at 95 °C for 40 min and blocking reagent Peroxidazed 1 (Biocare Medical). The primary antibodies used were anti-blood group A antigen (clone HE-193, dilution 1:50, cat. No. ab2521, Abcam, Cambridge, England), anti-blood group B antigen (HEB-29, 1:50, ab2524, Abcam), anti-H-type-1 (17–206, 1:50, ab3355, Abcam), and anti-GD1a ganglioside (GD1a-1, 1:50, MAB5606Z, Sigma-Aldrich, St. Louis, MO, USA). MACH 1 Universal HRP-polymer kit (Biocare Medical), including Betazoid DAB substrate and blocking reagent Background Sniper, was used for detection of bound antibodies. Nuclei were counterstained with Tacha’s automated hematoxylin (Biocare Medical).
Säljö K., Thornell A., Jin C., Stålberg P., Norlén O, & Teneberg S. (2021). Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands. International Journal of Molecular Sciences, 22(13), 7044.
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3

Immunohistochemical Analysis of p53 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed on 4 μm sections that were
cut from FFPE (formalin-fixed, paraffin-embedded) blocks. Sections were then
deparaffinised with xylene and rehydrated through graded ethanol. Antigen
retrieval was achieved with a pH 9.5 Nuclear Decloaker (Biocare Medical) in a
Decloaking pressure cooker at 95°C for 40 min. Tissue sections were then
treated with 3% hydrogen peroxide (LOT 161509; Fisher Chemical) and with
Background Sniper (Biocare Medical, Concord, CA, USA) to reduce nonspecific
background staining. Primary antibody for p53 (Cell Signaling, 2527) was applied
in a 1:150 dilution for 80 min followed by detection with the MACH 3 Rabbit HRP-
Polymer Detection kit (Biocare Medical). Visualization was achieved using VECTOR
NovaRED (SK-4800; Vector Laboratories, Inc.) as chromogen. Lastly, sections were
counterstained with Tacha's Automated Hematoxylin (Biocare Medical).
Mai W.X., Gosa L., Daniels V.W., Ta L., Tsang J.E., Higgins B., Gilmore W.B., Bayley N.A., Harati M.D., Lee J.T., Yong W.H., Kornblum H.I., Bensinger S.J., Mischel P.S., Rao P.N., Clark P.M., Cloughesy T.F., Letai A, & Nathanson D.A. (2017). Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nature medicine, 23(11), 1342-1351.
+ Open protocol
+ Expand

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