2000 ft ir spectrometer

FT-IR was carried out to identify the functional groups in GLP. Briefly, 2 mg of GLP was mixed with 200 mg of KBr into a 1 mm pellet. The FT-IR spectrum of GLP was recorded in the wave range of 400–4,000 cm-1 on a PerkinElmer 2,000 FT-IR spectrometer (Waltham, MA, USA).

¹H and ¹³C NMR spectra were recorded on a Bruker Avance 600 MHz instrument and referenced to residual solvent peaks. IR spectra were recorded with a PerkinElmer 2000 FT-IR spectrometer. Thermal analysis studies for 1 and 2 were performed on a TA Instruments SDT 650 analyzer at a heating rate of 2 °C min⁻¹ under dry nitrogen with a flow rate of 100 mL min⁻¹.

Infrared spectroscopy is a simple method for structure analysis. Samples were liophilized and milled with KBr to form a uniform capsule and were characterized via FT-IR spectroscopy on a Perkin Elmer 2000 FTIR spectrometer operated in the absorbance mode at a resolution of 4 cm^-1.

Chemicals were purchased from industrial manufacturers and, unless otherwise specified, were used without any further purification. Precoated Merck-silica gel 60F₂₅₄ plates were used for thin layer chromatography (TLC); UV cabinet was used to detect developed plates. Column chromatography was performed with 100–200 mesh silica gels. Melting points were recorded by Buchi M-560 instrument and were uncorrected. The IR spectroscopy was done with PerkinElmer 2000 FT-IR spectrometer; KBr disc were used for samples preparation. The ¹H NMR and ¹³C spectra were recorded on a Jeol alpha-400 and at 100.6 MHz, respectively, using TMS as an internal standard. The chemical shift values were on δ scale and the coupling constants (J) were in Hz. Signals from OH groups in ¹HNMR spectra were verified by removing them by shaking in D₂O.
High Resolution Mass Spectrometry (HRMS) was performed by AB SCIEX Triple TOFTM 5600+ equipped with Turboion Spray (TIS), SCIEX ExionLC, and PDA detector. Compounds were separated through C-18 column (2.7 µm, 4.6 × 100 mm) by eluting with methanol and water (98:2, v/v) at 0.3 ml/min at 40°C.
ED₅₀ values were estimated with the SPSS statistical package. The whole computational work was carried out by using VLifeMDS QSAR plus 4.6 software using the Lenovo PC having window 8.1 operating system and Intel (R) Celeron (R) processor.

The reactants and solvents were received from commercial suppliers and used without additional purification. All melting points are uncorrected and determined by the open capillary method using Apotec^® Schmelzpunktbestimmer melting point apparatus (WEPA Apothekenbedarf GmbH & Co. KG., Hillscheid, Germany). ¹H, ¹³C and ³¹P NMR spectra were collected at 25 °C using 400 MHz Jeol 400yh (Jeol Ltd., Tokyo, Japan) and 600 MHz Bruker Avance II 600 instruments (Bruker, Billerica, MA, USA). NMR spectra were measured in CDCl₃, unless otherwise stated. IR spectra were obtained either as potassium bromide pellets or as liquid films with a Perkin Elmer 2000 FTIR spectrometer (PerkinElmer, Waltham, MA, USA). High-resolution ESI mass spectra were obtained on a Waters LCT Premier XE TOF spectrometer (Waters Corporation, Milford, MA, USA). Optical rotations were determined at 25 °C with an Optical Activity Ltd. Model AA-5 automatic polarimeter (Optical Activity, Ltd., Ramsey, UK); [α]^D values are expressed in 10⁻¹ deg cm²·g⁻¹. Silica gel 60 was used for both column chromatography and thin-layer chromatography performed with the Merck plates (F254) which were visualized using UV light and iodine vapors.

All chemicals and anhydrous solvents were supplied by Aldrich Chemicals and used under a nitrogen atmosphere. FTIR measurements were carried out using a Perkin−Elmer 2000 FTIR spectrometer. UV−Vis absorption/emission spectra were recorded on a Horiba Scientific Duetta spectrophotometer/spectrofluorimeter equipped with EZSpec software (Kyoto, Japan). All excitation and emission spectra and time−correlated single photon counting (TCSPC) lifetimes were carried out using an Edinburgh Instruments FLS1000 photoluminescence spectrometer (Edinburgh, Scotland). For steady−state measurements, an Xe Arc lamp and a visible PMT−900 detector were used. For lifetimes, a 375 nm variable pulse length diode laser (VPL−375) was employed. All data analyses were carried out using Floracle ® software version 2.15.2. CO TONs were quantified through headspace sampling using a Shimadzu GC−2010 Plus gas chromatograph equipped with LabSolutions Lite 5.5 software (Kyoto, Japan). All NMR spectra (Figures S18−S30, Supporting Information) were recorded on a Bruker Avance Ultrashield 600 spectrometer with TopSpin 3.6.1 software and were referenced to the deuterated solvent peak as an internal reference. Elemental analyses were carried out at London Metropolitan University.

All solvents and chemicals were purchased from commercial sources (Sigma-Aldrich, Merck, and others) and used as received or dried using standard procedures. Melting points were determined on a Kofler apparatus and are uncorrected. Elemental analysis (CHN) has been conducted using a Thermo Scientific (FLASH 2000) CHN Elemental Analyser. Fourier transform-infrared (FT-IR) spectra were recorded using a Perkin-Elmer (2000 FTIR) Spectrometer by the KBr pellet method, values are given in cm⁻¹. The UV spectra were recorded with PerkinElmer UV Win Lab spectrophotometer. ¹H and ¹³C NMR spectra were run in CDCl₃ and DMSO-d₆ on Bruker Avance-II 400 and 100 MHz instruments, respectively. Mass spectra were recorded on a JEOL D-300 mass spectrometer. Thin-layer chromatography (TLC) glass plates were coated with silica gel (E-Merck G254) and exposed to iodine vapour to check the purity of the isolated compounds.