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Phospho foxo1 3 thr24 32

Manufactured by Cell Signaling Technology
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Phospho-FoxO1-3 (Thr24/32) is a primary antibody that specifically recognizes the phosphorylated forms of the FoxO1, FoxO3, and FoxO4 transcription factors at the threonine 24, 32, and 197 residues, respectively. This antibody is useful for detecting the activation status of the FoxO family of proteins.

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Lab products found in correlation

Total gsk3, by Cell Signaling Technology (2 mentions) Phospho gsk3 ser9, by Cell Signaling Technology (2 mentions) Phospho glycogen synthase ser641, by Cell Signaling Technology (2 mentions) Total akt1, by Cell Signaling Technology (2 mentions) Total akt2, by Cell Signaling Technology (2 mentions) Total glycogen synthase, by Cell Signaling Technology (2 mentions) Total foxo3, by Cell Signaling Technology (2 mentions) Appl1, by Cell Signaling Technology (2 mentions) Antibody to glut2, by Santa Cruz Biotechnology (2 mentions) Chemidoc xr, by Bio-Rad (2 mentions) Enhanced chemi luminescence (ecl), by Merck Group (2 mentions) Quantity one 1 d analysis software, by Bio-Rad (2 mentions) Phospho akt ser473, by Cell Signaling Technology (2 mentions) Phospho akt2, by Cell Signaling Technology (2 mentions) Phospho akt ser308, by Cell Signaling Technology (2 mentions) Myc tag, by Cell Signaling Technology (2 mentions) Total p70s6k, by Cell Signaling Technology (2 mentions) Phospho p70s6k thr389, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

FOXO1 (243 citations) Phospho-FoxO1 (19 citations)

2 protocols using phospho foxo1 3 thr24 32

1

Western Blot Analysis of Liver Proteins

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Proteins from total liver or cellular lysates or immunoprecipitated were separated by SDS-PAGE, and probed with different primary antibodies as specified in each figure legend. The specific signals were amplified by addition of horseradish peroxidase-conjugated secondary antibodies and visualized with enhanced chemiluminescence (ECL from Millipore, MA, USA). Western blotting images were processed using a ChemiDoc XRS digital imaging system with Quantity One 1-D analysis software (Bio-Rad Laboratories, Inc., Hercules, CA, USA)
Antibodies from Cell Signaling Technology (working dilution 1:1000) were the following: phospho-Akt (Ser473) (#4060), phospho-Akt (Ser308) (#13038), phospho-Akt2 (#8599) phospho-GSK3 (Ser9)(#9327), phospho-FoxO1-3 (Thr24/32) (#9464), phospho-p70S6K (Thr389) (#9234), phospho-Glycogen Synthase (Ser641) (#3891), total Akt1 (#2967), total Akt2 (#3063, #5239), total Glycogen Synthase (#3893), total FoxO3 (#12829), total GSK3 (#12456), total p70S6K (#2708), APPL1(#3858), Rab5(#2143) (#3547), Rab7(#2094), Myc-Tag (#2272). Antibody to Glut2 (working dilution 1:1000) was from Santa Cruz biotechnology Inc (sc-9117). Original gel images are shown in Supplementary Fig. 9.
Braccini L., Ciraolo E., Campa C.C., Perino A., Longo D.L., Tibolla G., Pregnolato M., Cao Y., Tassone B., Damilano F., Laffargue M., Calautti E., Falasca M., Norata G.D., Backer J.M, & Hirsch E. (2015). PI3K-C2γ is a Rab5 effector selectively controlling endosomal Akt2 activation downstream of insulin signalling. Nature Communications, 6, 7400.
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2

Western Blot Immunodetection Protocol

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Proteins from total liver or cellular lysates or immunoprecipitated were separated by SDS– polyacrylamide gel electrophoresis (SDS–PAGE), and probed with different primary antibodies as specified in each figure legend. The specific signals were amplified by addition of horseradish peroxidase-conjugated secondary antibodies and visualized with enhanced chemiluminescence (ECL from Millipore). Western blotting images were processed using a ChemiDoc XRS digital imaging system with Quantity One 1-D analysis software (Bio-Rad Laboratories, Inc.).
Antibodies from Cell Signaling Technology (working dilution 1:1,000) were the following: phospho-Akt (Ser473) (#4060), phospho-Akt (Ser308) (#13038), phospho-Akt2 (#8599) phospho-GSK3 (Ser9)(#9327), phospho-FoxO1-3 (Thr24/32) (#9464), phospho-p70S6K (Thr389) (#9234), phospho-Glycogen Synthase (Ser641) (#3891), total Akt1 (#2967), total Akt2 (#3063, #5239), total Glycogen Synthase (#3893), total FoxO3 (#12829), total GSK3 (#12456), total p70S6K (#2708), APPL1(#3858), Rab5(#2143) (#3547), Rab7(#2094), Myc-Tag (#2272). Antibody to Glut2 (working dilution 1:1,000) was from Santa Cruz Biotechnology Inc (sc-9,117). Original gel images are shown in Supplementary Fig. 9.
Braccini L., Ciraolo E., Campa C.C., Perino A., Longo D.L., Tibolla G., Pregnolato M., Cao Y., Tassone B., Damilano F., Laffargue M., Calautti E., Falasca M., Norata G.D., Backer J.M, & Hirsch E. (2015). PI3K-C2γ is a Rab5 effector selectively controlling endosomal Akt2 activation downstream of insulin signalling. Nature Communications, 6, 7400.
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