Anti rabbit igg sa00001 2

Manufactured by Proteintech

Sourced in China, United States

Anti-rabbit IgG (SA00001-2) is a secondary antibody that binds to rabbit primary antibodies. It is used in various immunoassays and detection techniques to amplify the signal from rabbit-derived primary antibodies.

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Lab products found in correlation

Anti mouse igg sa00001 1, by Proteintech (4 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Anti lc3a b, by Cell Signaling Technology (1 mentions) Rapamycin rapa, by MedChemExpress (1 mentions) Alexa fluor 488 conjugated goat anti rabbit igg, by Beyotime (1 mentions) Chloroquine cq, by Merck Group (1 mentions) Acridine orange, by Solarbio (1 mentions) Coomassie blue r 250, by Merck Group (1 mentions) Histrap hp column, by Cytiva (1 mentions) Pd 10 desalting column, by Cytiva (1 mentions) Horseradish peroxidase hrp conjugated anti mouse immunoglobulin g igg sa00001 1, by Proteintech (1 mentions) Mouse anti his monoclonal antibody m0812 3, by HuaAn Biotechnology (1 mentions) Polyvinylidene fluoride pvdf membrane, by Beyotime (1 mentions) Anti rabbit igg labeled with alexa fluor 488 a11034, by Thermo Fisher Scientific (1 mentions) Beta actin ab8226, by Abcam (1 mentions) Imaging software, by Merck Group (1 mentions) Chemiluminescence, by Merck Group (1 mentions) Anti p21 10355 1 ap, by Proteintech (1 mentions) Anti cd11b 66519 1 ig, by Proteintech (1 mentions) Anti β actin, by Proteintech (1 mentions)

12 protocols using anti rabbit igg sa00001 2

Chloroquine and Rapamycin Autophagy Modulation

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Chloroquine (CQ) was purchased from Sigma-Aldrich (St. Louis, MI, USA). Rapamycin (Rapa) was purchased from MedChemExpress, (South Brunswick, NJ, USA). Acridine orange (AO) was purchased from Solarbio (Beijing, China). EG was purchased from Macklin (Shanghai, China).
The antibodies used in this study were anti-Fragilis (Huabio, Hangzhou, China), anti-LC3A/B (Cell Signaling Technology, Danvers, MA, USA), anti-cathepsin B polyclonal antibody (Wanleibio, Shenyang, China), anti-BVDV E2-specific mouse monoclonal antibody (VMRD, Pullman, WA, USA). Additionally, anti-LAMP1 (21997-1-AP), anti-SQSTM1/p62 (18420-1-AP), anti-GAPDH (60004-1-AP), and anti-Tubulin (10068-1-AP) were purchased from ProteinTech Group Inc. (Rosemont, IL, USA). Secondary antibodies in this study included Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG (Beyotime, Shanghai, China). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (SA00001-1), and anti-rabbit IgG (SA00001-2) were purchased from ProteinTech Group Inc. (Rosemont, IL, USA).

Zhang L., Yang G., Wang J., Zhang J., Chen K., Xiong X., Zhu Y., Xu C, & Wang J. (2023). Ethyl Gallate Inhibits Bovine Viral Diarrhea Virus by Promoting IFITM3 Expression, Lysosomal Acidification and Protease Activity. International Journal of Molecular Sciences, 24(10), 8637.

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Protein Purification and Antibody Detection

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Rabbit anti-HA polyclonal antibody (0906-1) and mouse anti-His monoclonal antibody (M0812-3) were purchased from Hangzhou HuaAn Biotechnology Co. (Hangzhou, China). Horseradish peroxidase (HRP)-conjugated anti-mouse immunoglobulin G (IgG) (SA00001-1) and anti-rabbit IgG (SA00001-2) were purchased from the ProteinTech Group (Chicago, IL, USA). HisTrap HP columns and PD-10 desalting columns were purchased from Amersham Pharmacia Biotech, Inc. (Piscataway, NJ, USA). Anti-FLAG antibody (F3165), mouse anti-HA monoclonal antibody (H9658), control IgG (M5284) and Coomassie blue R-250 were purchased from Sigma (St. Louis, MO, USA).

YOU Q., WU Y., YAO N., SHEN G., ZHANG Y., XU L., LI G, & JU C. (2015). Interaction of AIM with insulin-like growth factor-binding protein-4. International Journal of Molecular Medicine, 36(3), 833-838.

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Extraction and Biochemical Assays for JFP-Ps

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The JFP-Ps were extracted, purified and prepared according to our previous method at the Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences [9 (link)].
Malonic dialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) assay kits were purchased from Suzhou Geruisi Biotechnology Co., Ltd. (Suzhou, China). Tumor necrosis factor alpha (TNF-α), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-10 (IL-10) and interferon gamma (IFN-γ) assay kits were purchased from Shanghai Enzyme-linked Biology Co., Ltd. (Shanghai, China). Primers were purchased from Shenggong Biotechnology Co., Ltd. (Shanghai, China). Polyclonal antibodies of β-actin (20536-1-AP), IκBα (10268-1-AP), nuclear factor kappa-B (NF-κB) p65(10745-1-AP), p38 MAPK (14064-1-AP), JNK (24164-1-AP) and anti-rabbit IgG (SA00001-2) were purchased from Proteintech Group, Inc. (Wuhan, China). Phospho-p38 MAPK (p-p38, AP0526) was purchased from ABclonal, Inc. (Wuhan, China), phospho-JNK (p-JNK, ab76572) was purchased from Abcam (Shanghai) Trading Co., Ltd. (Shanghai, China). Phospho-NF-κB p65 (p-p65, AF5875) and polyvinylidene fluoride (PVDF) membrane were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

Cheng M., Zheng Y., Wu G., Tan L., Xu F., Zhang Y., Chen X, & Zhu K. (2024). Protective Effect of Artocarpus heterophyllus Lam. (Jackfruit) Polysaccharides on Liver Injury Induced by Cyclophosphamide in Mice. Nutrients, 16(1), 166.

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Antibody Characterization for TGF-β Signaling

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Anti-PDCoV N antibody was purchased from Medgene labs (Brookings, SD, USA), anti-TGF-β1 (WL02998), anti-Smad3 (WL02288), anti-phospho-Smad3/Smad2 (WL02305), and anti-TGF-βRI (WL03150) were ordered from Wanleibio (Shenyang, China), and anti-β-actin (60008-1-AP), antitubulin (10068-1-AP), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (SA00001-1), and anti-rabbit IgG (SA00001-2) were ordered from Proteintech (Wuhan, China). Anti-mouse IgG antibody labeled with Alexa Fluor 555 (A21424) and Alexa Fluor 488 (A21429) and anti-rabbit IgG labeled with Alexa Fluor 488 (A11034) were ordered from Thermo Fisher Scientific. The aforementioned antibodies were used at dilutions of 1:300 for immunofluorescence staining and 1:1,500 for immunoblotting analysis.

Zhang J., Yang G., Wang X., Zhu Y, & Wang J. (2022). 25-Hydroxycholesterol Mediates Cholesterol Metabolism to Restrict Porcine Deltacoronavirus Infection via Suppression of Transforming Growth Factor β1. Microbiology Spectrum, 10(6), e02198-22.

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Protein Expression and Analysis

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Recombinant porcine FSH (Sigma F2293), recombinant human IGF1 (ab270062), APLN-13 (ab141010), APLN-17 (ab141011) were purchased from Abcam. Also, Bax (ab77566), and beta-Actin (ab8226) antibodies were obtained from Abcam. Anti-rabbit IgG (SA00001-2), and anti-mouse IgG (SA00001-1) were purchased from Proteintech. Primary and secondary antibodies were used at 1: 500 to 1:1000 and 1/3000 for western blotting.

Shokrollahi B., Zheng H.Y., Ma X.Y, & Shang J.H. (2023). The effects of apelin on IGF1/FSH-induced steroidogenesis, proliferation, Bax expression, and total antioxidant capacity in granulosa cells of buffalo ovarian follicles. Veterinary research communications, 47(3).

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Protein Isolation and Western Blot Analysis

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After the experiment, the brain tissues and pancreas tissues of rats were collected, and the total proteins were extracted by radio immunoprecipitation analysis (RIPA) and lysis buffer solution. The protein concentration was determined by bicinchoninic acid (BCA) method. A total of 200 μg protein samples were separated by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The isolated proteins were transferred to a polyvinylidene fluoride membrane that had been activated by methanol and blocked by 5% skim milk and dried at room temperature for at least 1 h. The primary antibodies were then incubated overnight at 4°C. The primary antibodies for incubation included anti-CD11b (66519-1-Ig, 1:2000, Proteintech, USA), anti-CD86 (13395-1-AP, 1:1000, Proteintech, USA), anti-cleaved-caspase 3 (19677-1-AP, 1:1000, Proteintech, USA), anti-p21 (10355-1-AP, 1:1000, Proteintech, USA), anti-P16 (10883-1-AP, 1:1000, Bioss, China), and anti-b-actin (60008-1-Ig, 1:5000, Proteintech, USA). They were then incubated with anti-mouse IgG (SA00001-1, 1:5000, Proteintech, USA) and antirabbit IgG (SA00001-2, 1:6000, Proteintech, USA) at 37 °C for 90 min. Chemiluminescence (Millipore, USA) was visualized and analysed using imaging software (GE Healthcare, Life Sciences, USA).

Li X., He J., Li X., Li Y., Zhou Y, & Cai S. (2021). Neu-P11 - a novel melatonin receptor agonist, could improve the features of type-2 diabetes mellitus in rats. Endokrynologia Polska, 72(6).

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Protein Expression Analysis of Pancreatic Tissue

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Pancreatic tissue (∼30 mg) was homogenized in RIPA lysis buffer (Beyotime Biotechnology) mixed with a protease and phosphatase inhibitor cocktail (ab201119, Abcam). Total protein was purified by centrifugation, and the concentration was determined by means of the BCA kit. An aliquot of the supernatant containing 30 to 60 μg total protein were subjected to standard Western blotting procedures. Immunoblotting analysis was performed with antibodies against CEL (15384-1-AP, Proteintech), Flag (AT0022, CMCTAG), CHOP (also known as DDIT3) (#2895, Cell Signaling Technology), XBP-1s (#12782, Cell Signaling Technology), BiP (also known as HSPA5) (#3177, Cell Signaling Technology), Beclin1 (11306-1-AP, Proteintech), P62 (#39749, Cell Signaling Technology), LC3 (#12741, Cell Signaling Technology), β-actin (66009-1-Ig, Proteintech) and secondary antibodies (anti-mouse IgG SA00001-1 or anti-rabbit IgG SA00001-2, Proteintech). Immunoblotting analysis of CEL proteins in different fractions of transfected HEK293T cells was performed with another antibody against CEL (HPA052701, Sigma). Densitometric analysis was carried out using Image J software.

Mao X.T., Zou W.B., Cao Y., Wang Y.C., Deng S.J., Cooper D.N., Férec C., Li Z.S., Chen J.M, & Liao Z. (2022). The CEL-HYB1 Hybrid Allele Promotes Digestive Enzyme Misfolding and Pancreatitis in Mice. Cellular and Molecular Gastroenterology and Hepatology, 14(1), 55-74.

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Co-Immunoprecipitation of p75NTR and Sortilin

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BMSCs were lysed using IP lysis buffer (AWB0144, Abiowell, China), and the protein-containing supernatant was collected post-centrifugation. The protein extract was allocated into four separate tubes, to which specific antibodies were added: anti-p75NTR (55014-1-AP, 1:1000, Proteintech), anti-sortilin (12369-1-AP, 1:8000, Proteintech), or anti-rabbit IgG (SA00001-2, 1:6000, Proteintech), according to the experimental requirements. After thorough mixing, the mixtures were incubated overnight. The antibody-protein complexes were then combined with pre-treated Protein A/G agarose beads and incubated with gentle agitation at 4℃ for 2 h, followed by centrifugation. The beads, which have bonded with the antibody-protein complexes, were retained, and the supernatant was discarded after washing the beads four times with IP lysis buffer. Lastly, IP lysis buffer and 5× loading buffer were added to the bead-bound complexes, which were then mixed, denatured, and prepared for subsequent protein quantification analysis.

Wu Z., Yang Y, & Wang M. (2024). Silencing p75NTR regulates osteogenic differentiation and angiogenesis of BMSCs to enhance bone healing in fractured rats. Journal of Orthopaedic Surgery and Research, 19, 192.

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Naringenin Modulates Inflammatory Pathways

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Naringenin (must-21032406) was purchased from CDMUST (Chengdu, China). Other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-alpha smooth muscle actin (α-SMA) (A7248), anti-cGAS (A8335), anti-IRF3 (A2172), anti-P-TBK1 (AP0847), anti-TBK1 (A3458), anti-IL8(A12452), and anti-STING (A20175) were purchased from ABclonal (Woburn, MA, USA). Anticollagen I (14695-1-AP), anti-β-actin (66009-1-Ig), anti-rabbit IgG (SA00001-2), and anti-mouse IgG (SA00001-1) were purchased from Proteintech Group (Rosemont, IL, USA). Anti-IL1β (ab234437), anti-IL6 (ab229381), and anti-TNFα (ab215188) were purchased from Abcam (Cambridge, UK).

Chen L., Xia S., Wang S., Zhou Y., Wang F., Li Z., Li Y., Kong D., Zhang Z., Shao J., Xu X., Zhang F, & Zheng S. (2022). Naringenin is a Potential Immunomodulator for Inhibiting Liver Fibrosis by Inhibiting the cGAS-STING Pathway. Journal of Clinical and Translational Hepatology, 11(1), 26-37.

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YQHX Modulates Endothelial Cell Function

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Endothelial cell culture media (ECM), endothelial cell growth supplies (ECGS), and fetal bovine serum (FBS) were purchased from ScienCell Research Laboratories (ScienCell, CA, USA) or Gibco (Gibco, CA, USA). All of the chemicals, including LPS, simvastatin (ST), pyrrolidine dithiocarbamate (PDTC), and 3-(4,5-dimethyl-2-thiazolyl)-2, and 5-diphenyl-2-H-tetrazolium bromide (MTT) dye, were purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibodies against PAI-1 (sc-5297), TF (sc-393657), KLF2 (sc-28675), p65 (sc-109), p-p65 (sc-33020), IκB (sc-371), p-IκB (sc-8404), GAPDH (sc-47724), and β-actin (sc-47778) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase- (HRP-) conjugated anti-mouse IgG (SA00001-1) and anti-rabbit IgG (SA00001-2) antibodies were purchased from Proteintech Biotechnology (Proteintech Ltd., Wuhan, China). Lipofectamine 2000 was obtained from Thermo Fisher Scientific (Thermo, MA, USA). YQHX, composed of Ginseng Radix et Rhizoma, Astragali Radix, Paeoniae Rubra Radix, and Carthami Flos, was produced by Sichuan Neo-Green Pharmaceutical Technology Development Co., Ltd. (Sichuan, China). It was freshly prepared in a phosphate-buffered solution before use.

Wu H., Wang X., Gao S., Dai L., Tong H., Gao H., Lei Z., Han Y., Wang Z., Han L, & Qi D. (2019). Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation. Oxidative Medicine and Cellular Longevity, 2019, 9425183.

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