Anti-mouse CD152 (anti-CTLA-4) (Clone 9H10, GoInVivo™, BioLegend, CA, USA) was administered i.p. at 5μg/g mouse body weight on days 8, 11, and 14 in 100μL PBS.(21 (link)) Anti-mouse CD279 (anti-PD-1) antibody (Clone RMP1-14, GoInVivo™, BioLegend, CA, USA) was administered i.p. at 5μg/g mouse body weight on days 8, 11, and 14 in 100μL PBS. Anti-IFN-γ antibody (Clone XMG1.2, BioXCell, NH, USA) was administered i.p. at 25μg/g mouse in 200μL PBS. (22 (link)) Corresponding isotype controls were used: mouse IgG1 Isotype Ctrl Antibody and rat IgG2a Isotype Ctrl Antibody (BioLegend, GoInVivo™). 1-methyl-D-tryptophan (Sigma-Aldrich, MO, USA) was administered ad lib in drinking water of mice as previously described.(14 (link)) Mice drank approximately 2.5–3.5mL of 1-D-MT supplemented water per day. Epacadostat (Selleckchem) was administered 300mg/kg.(23 (link))
Brown Z.J., Yu S.J., Heinrich B., Ma C., Fu Q., Sandhu M., Agdashian D., Zhang Q., Korangy F, & Greten T.F. (2018). Indoleamine 2,3-dioxygenase provides adaptive resistance to immune checkpoint inhibitors in hepatocellular carcinoma. Cancer immunology, immunotherapy : CII, 67(8), 1305-1315.
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