Ncl l ll002

Manufactured by Abcam

NCL-L-LL002 is a monoclonal antibody that can be used for the detection of Laminin-5 in immunohistochemical applications. Laminin-5 is a component of the extracellular matrix and plays a role in cell adhesion.

Automatically generated - may contain errors

Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti c kit, by Agilent Technologies (1 mentions) Anti cldn1, by Santa Cruz Biotechnology (1 mentions) Anti dsp, by Santa Cruz Biotechnology (1 mentions) Anti cdh1, by Cell Signaling Technology (1 mentions) Prb 417p, by Fortrea (1 mentions) Fluoromount, by Merck Group (1 mentions)

Spelling variants of this product

LL002 (4 citations)

2 protocols using ncl l ll002

Comprehensive Immunomarker Panel for Epidermal Characterization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies included anti-PERP (N-terminal, Atlas HPA022269; C-terminal, previously reported (Franke et al., 2013 (link))), anti-c-Kit (1:500, Dako A4502), anti-Ki67 (Novocastra RTU-Ki67-MM1), anti-KRT14 (1:2000, Novocastra NCL-L-LL002), anti-KRT1 (1:800, Abcam ab83664), anti-FLG (1:300, Covance PRB-417P), anti-LOR (1:500, Covance PRB-145P) anti-IVL (1:400, Sigma I 9018), anti-DSG1 (immunoblotting, Santa Cruz sc-20114; immunocytochemistry, Abcam ab122913), anti-DSG3 (Life Technologies 32–6300), anti-DSC3 (Progen 6J193), anti-JUP (Santa Cruz sc-8415), anti-DSP (Santa Cruz sc-33555), anti-CDH1 (Cell Signaling 3195), anti-CLDN1 (immunoblotting Santa Cruz sc-166338; immunocytochemistry Abcam ab15098) and anti-cleaved-caspase3 (1:50 Cell Signaling 9661).

Duchatelet S., Boyden L.M., Ishida-Yamamoto A., Zhou J., Guibbal L., Hu R., Lim Y.H., Bole-Feysot C., Nitschké P., Santos-Simarro F., de Lucas R., Milstone L.M., Gildenstern V., Helfrich Y.R., Attardi L.D., Lifton R.P., Choate K.A, & Hovnanian A. (2018). Mutations in PERP cause dominant and recessive keratoderma. The Journal of investigative dermatology, 139(2), 380-390.

+ Open protocol

+ Expand

Immunohistochemical Identification of Rare Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

In preparation for selection, tissue sections were stained by immunohistochemistry (IHC) with antibodies against K19 and K14 and detected with Alexa Flour A568 and A488 conjugated isotype specific goat anti-mouse antibodies, respectively. For details on the antibodies, see Tables 1 and 2.Table 1

Primary antibodies used for IHC

Description	Isotype	Dilution	Source
Mouse monoclonal Anti-K14	IgG3	1:50	Leica Biosystems, NCL-L-LL002
Mouse monoclonal Anti-K19	IgG2a	1:100	Abcam, ab7754
dAb	-	~250 μg/mL	-
dAb-rFc	-	40–100 μg/mL	-

Table 2

Secondary antibodies used for IHC

Description	Flourophore	Isotype specificity	Dilution	Source	Detection of
Goat anti-mouse	A488	IgG₃	1:500	Invitrogen, A21151	CK14
Goat anti-mouse	A350	IgG_2a	1:250	Invitrogen, A21130	CK19
Goat anti-mouse	A488	IgG_2a	1:500	Invitrogen, A21131	CK19
Mouse anti-human c-Myc	Cy3	IgG₁	1:250	Sigma-Aldrich, C6594	dAb
Goat anti-rabbit	A488	IgG (H + L)	1:400	Invitrogen, A11008	dAb-rFc

A Alexa Fluor^® fluorescent dye

One tissue section from each patient was mounted with Fluoromount (Sigma-Aldrich), and areas containing rare K19⁺/14⁺ cells were identified. Only patient samples containing 1–2 areas with 2–10 luminal positioned K19⁺/14⁺ cells residing in ductal regions were used for selections.

Sørensen K.M., Meldgaard T., Melchjorsen C.J., Fridriksdottir A.J., Pedersen H., Petersen O.W, & Kristensen P. (2017). Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections. BMC Cancer, 17, 19.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!